-
Plasmid#197908Purposesingle-particle tracking for mTBPDepositorInsertTBP (Tbp Mouse)
UseTagsHalotagExpressionMammalianMutationPromoterEF1Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAL3
Plasmid#114673PurposeT7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.DepositorTypeEmpty backboneUseTagsoptional Ser-Gly-6HisExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
AB65_pCAG.Cas9-D10A.rBGpA
Plasmid#199256PurposeExpression construct encoding the SpCas9-D10A nickaseDepositorInsertSpCas9.D10A nickase
UseCRISPRTagsSV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PBDGTV
Plasmid#100859PurposeA piggyBac transposon based dual directional gene trap vector, in which, the mutagens are two non-selectable gene-trap cassettes in opposite orientations.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP161
Plasmid#189536PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFPDepositorInsertsRNase III
gUTR-161-GFP
UseTagsExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
AV11_pDonor.EP.S1
Plasmid#100296PurposeAAVS1-targeting expression plasmidDepositorInsertPuroR.T2A.EGFP expression unit
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJin-264
Plasmid#125717PurposeABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19DepositorInsertPT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
hGR-Dx
Plasmid#133408Purposeoverexpression of a mutant human glucocorticoid receptor in mammalian cellsDepositorInsertNR3C1 (NR3C1 Human)
UseTagsExpressionMammalianMutationN454D, A458T, R460D, D462CPromoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZH051
Plasmid#47311PurposeExpresses Tsr-Venus-Ubiquitin-CIDepositorInsertwild type immunity region of lambda phage with tsr-venus-ub-cI
UseTagsCIExpressionBacterialMutationPromoterPR; PRMAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJH579
Plasmid#172163PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD JM10.1); AraC
UseTagsExpressionBacterialMutationSumo(S6L) PylB(N61S, E84K, E122K, E175K, E178K, G…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 arr
Plasmid#112890PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertarr
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 fosA
Plasmid#112889PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertfosA
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJin-239
Plasmid#125716PurposeABA-inducible split T7 gRNA for GFP vector using RNAPN N-29-1DepositorInsertpT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (N-29-1)-linker-ABI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS1
Plasmid#55749PurposeProduces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
UseTagsExpressionBacterialMutationPromoterT7-lacAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
DPY-pNCS
Plasmid#17365DepositorInsertMMLV proviral DNA
UseTagsExpressionMutationdeletion that removes 5 amino acids (DPPPY) from …PromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAGH51
Plasmid#107255PurposeHU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.DepositorInsertHU-FRB
UseSynthetic BiologyTagsFRBExpressionMutationPromoternative HU promoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAWV
Plasmid#62710PurposeDonor vector for amiR-eGFP knock-in via landing padDepositorInserttdTomato/amiR-eGFP123/amiR-eGFP419/FLPe
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
dSp-Rta Full (1-190)
Plasmid#99662PurposeExpresses dSp Cas9 fused to truncated RTA activation domainDepositorArticleInsertdCas9
UseTagstruncated RTA activation domain (1-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYG205
Plasmid#200836PurposeTranscribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivativesDepositorInsertrapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP-BADAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRnc2-gGFP
Plasmid#189563PurposeExpresses E. Coli RNase III under control of pBAD promoter with native 5' untranslated region and constitutively expresses gUTR-129-GFPDepositorInsertsRNase III
gUTR-129-GFP
UseTagsExpressionBacterialMutationMaintains 5' UTR of rncPromoterJ23119 and pBADAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only