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Plasmid#107255PurposeHU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.DepositorInsertHU-FRB
UseSynthetic BiologyTagsFRBExpressionMutationPromoternative HU promoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAWV
Plasmid#62710PurposeDonor vector for amiR-eGFP knock-in via landing padDepositorInserttdTomato/amiR-eGFP123/amiR-eGFP419/FLPe
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
dSp-Rta Full (1-190)
Plasmid#99662PurposeExpresses dSp Cas9 fused to truncated RTA activation domainDepositorArticleInsertdCas9
UseTagstruncated RTA activation domain (1-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pYG205
Plasmid#200836PurposeTranscribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivativesDepositorInsertrapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP-BADAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRnc2-gGFP
Plasmid#189563PurposeExpresses E. Coli RNase III under control of pBAD promoter with native 5' untranslated region and constitutively expresses gUTR-129-GFPDepositorInsertsRNase III
gUTR-129-GFP
UseTagsExpressionBacterialMutationMaintains 5' UTR of rncPromoterJ23119 and pBADAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP129
Plasmid#189537PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-129-GFPDepositorInsertsRNase III
gUTR-129-GFP
UseTagsExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKP925
Plasmid#167591PurposeExpress TrpC-TonB fusion proteinDepositorInserttrpC-tonB
UseTagsExpressionBacterialMutationResidues 1-25 TrpC; Residues 26-31 Linker; Residu…PromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH858
Plasmid#172176PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH859
Plasmid#172177PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH580
Plasmid#172164PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2v2+B); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D, G332…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH581
Plasmid#172165PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2+C); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH750
Plasmid#172170PurposeMicroscopy inclusion body assayDepositorInsertpCDF.3f2, deleted sumo
UseTagsExpressionBacterialMutationEvolved PylBCD operon; SUMO tag deleted from PylB…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH751
Plasmid#172171PurposeMicroscopy inclusion body assayDepositorInsertpCDF.JM10.1, deleted sumo
UseTagsExpressionBacterialMutationEvolved PylBCD operon; SUMO tag deleted from PylB…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH768v2
Plasmid#172173PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.3f2]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH769v2
Plasmid#172174PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDM_N1_400
Plasmid#170474PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, O-, O1, Lambda t1 in modified lacZ fragment
UseTagsExpressionBacterialMutationdiminished hairpins near O1 and diminished latent…PromoterT7A1Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgiRFP1
Plasmid#113972PurposeSingle short guide RNA targeting GATCGAGTTCGAGCCTGCGG in iRFP670 sequenceDepositorInsertiRFP670 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgiRFP2
Plasmid#113973PurposeSingle short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequenceDepositorInsertiRFP670 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgiRFP3
Plasmid#113974PurposeSingle short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequenceDepositorInsertiRFP670 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only