Addgene

Showing: 321 - 340 of 3652 results

Results per page:

Sort results by:

pCMV-PEmax-SpG-P2A-hMLH1dn

Plasmid

#211550

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

Insert

PEmax-SpG-P2A-hMLH1dn

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

Plasmid

#194063

Purpose

Mammalian expression of evoCjCas9 PEmax_deltaRnH

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-PEmax_dRnH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-evoCjCas9-BE4max-BlastR

Plasmid

#194061

Purpose

Mammalian expression of evoCjCas9 CBE4max

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-BE4max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

Plasmid

#196829

Purpose

Express dpdpCas13b with AAAA mutation and Clover

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

dpspCas13b(AAAA)

Use

Tags

3XFLAG and NES

Expression

Mammalian

Mutation

K367A, D369A and K370A

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3TS-spCas9-ABE-Umax-rest1

Plasmid

#222144

Purpose

Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-miniSdd7-BE4max-BlastR

Plasmid

#204851

Purpose

For cytosine base editing using miniSdd7 in mammalian cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd7-nSpCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CMV, SV40, EM7

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-eA3Amax-T31A-NG-BlastR

Plasmid

#153000

Purpose

C-to-T/G/A base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T31A-nSpCas9-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-BE4max-P2A-EGFP (pRZ33)

Plasmid

#140083

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

Depositor

Keith Joung

Insert

Human codon optimized pCAG BE4max with P2A-EGFP

Use

Tags

P2A-EGFP

Expression

Mammalian

Mutation

D10A (SpCas9)

Promoter

CAG

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-miniSdd6-BE4max-BlastR

Plasmid

#204850

Purpose

For cytosine base editing using miniSdd6 in mammalian cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd6-nSpCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CMV, SV40, EM7

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SECURE miniABEmax-K20A/R21A (pJUL1774)

Plasmid

#131312

Purpose

CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Cas12i-Max-T2A-EGFP

Plasmid

#188268

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i-Max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-HNHx-ABEmax7.10HD-BlastR

Plasmid

#176477

Purpose

A-to-G base editor with alternative targeting scope

Depositor

Gerald Schwank

Article

Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

Insert

HNHx-ABEmax7.10HD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-His-Max-A-PQBP1-Y65C

Plasmid

#25803

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Apr 21. ():.

Insert

PQBP1 with Y65C mutation (PQBP1 Human)

Use

Tags

His and Xpress

Expression

Mammalian

Mutation

Tyrosine 65 within the WW domain of PQBP1 is chan…

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-ABEmax(TadA, TadA*E59A)

Plasmid

#125662

Purpose

pCMV-ABEmax(TadA, TadA*E59A) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* E59A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-T7Max-UTR1-CTerminus8xHis-T500

Plasmid

#178419

Purpose

Empty backbone. T7Max promoter with C-His Tag

Depositor

Kate Adamala

Article

Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Tags

8xHis

Expression

Bacterial

Mutation

Promoter

T7Max

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/HisMaxB-YAP2-1st WW mutant

Plasmid

#18998

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine, and Proline 2…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA4/HisMaxB-YAP2-2nd WW mutant

Plasmid

#18999

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Tryptophan 258 changed to Alanine, and Proline 2…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-T7-PEmax-P2A-EGFP (LM1589)

Plasmid

#223136

Purpose

pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized PEmax-P2A-EGFP

Use

CRISPR

Tags

BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP

Expression

Mammalian

Mutation

M-MLV mutations from PE2 (Anzalone et al. Nature …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-EA-evoAPOBEC1max-32NLS_NG-BlastR

Plasmid

#232722

Purpose

C-to-T base editor

Depositor

David Liu

Insert

evoAPOBEC1max H47E+S48A-32NLS linker-nCas9(NG)-UGI-UGI

Use

Tags

Expression

Mammalian

Mutation

within evoAPOBEC1: H47E+S48A; within Cas9 D10A

Promoter

Available since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3TS-spCas9 ABE-Umax-ex1

Plasmid

#222141

Purpose

Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge