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rescue plasmid

Plasmid

#135971

Purpose

expresses copGFP and puromycin resistance genes

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_045

Plasmid

#107144

Purpose

cloning of a sgRNA

Depositor

David Root

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEC1.2/EC1.1_spCas9_GFP

Plasmid

#161933

Purpose

CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection

Depositor

Christian Fankhauser

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEC1.2/EC1.1_spCas9_TagRFP

Plasmid

#161934

Purpose

CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection

Depositor

Christian Fankhauser

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pXPR_016

Plasmid

#107143

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

David Root

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFGC-pcoCas9

Plasmid

#52256

Purpose

A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

Depositor

Jen Sheen

Insert

35SPPDK:pcoCas9:NOS cassette

Use

CRISPR; Plant expression

Tags

Expression

Mutation

Promoter

constitutive 35SPPDK promoter

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pWS082

Plasmid

#90516

Purpose

sgRNA entry vector

Depositor

Tom Ellis

Insert

Yeast sgRNA expression vector

Use

CRISPR; Yeast gap repair

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pUC119-MCS

Plasmid

#58807

Purpose

contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.

Depositor

Jen Sheen

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pU6-Universal

Plasmid

#52694

Purpose

This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

Depositor

Sebastian Lourido

Inserts

Cas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA

Use

CRISPR; Toxoplasma gondii

Tags

3X FLAG and Nuclear localization signal

Expression

Mutation

Promoter

TgTUB1 and Toxoplasma U6

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pEC1.2/EC1.1_Cas9i_TagRFP

Plasmid

#227249

Purpose

CRISPR plasmid with Cas9i expressed under egg cell promoter - TagRFP seed selection

Depositor

Christian Fankhauser

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

pEC1.2/EC1.1

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

Plasmid

#107055

Purpose

Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

Depositor

Alex Hewitt

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_047

Plasmid

#107145

Purpose

measures Cas9 activity

Depositor

David Root

Insert

GFP + sgRNA for GFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_118

Plasmid

#113667

Purpose

Lentiviral vector expressing dCas9

Depositor

John Doench

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

catalytically inactive

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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MLM3636

Plasmid

#43860

Purpose

guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

Depositor

Keith Joung

Insert

None

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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pML104-KanMx4

Plasmid

#83476

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

Depositor

Patricia Wittkopp

Insert

KanMX4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pML104-HygMx4

Plasmid

#83475

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

Depositor

Patricia Wittkopp

Insert

HphMX4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pML104-NatMx3

Plasmid

#83477

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.

Depositor

Patricia Wittkopp

Insert

NatMx3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only
pX552-GFP

Plasmid

#107023

Purpose

pX552-CMV-GFP. AAV plasmid for sgRNA cloning, with GFP fusion facilitating FACS sorting of cells. Replaced the hSyn promoter in pX552 from Zhang lab with a CMV promoter.

Depositor

Alex Hewitt

Insert

GFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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BPK1520_blastR

Plasmid

#175289

Purpose

Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection

Depositor

Ronald Cohn

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV/U6

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-US-ECasE

Plasmid

#83961

Purpose

Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

Depositor

Eleanor Chen

Insert

ERT2-spCas9-ERT2

Use

Tags

Expression

Mammalian

Mutation

please see depositor comments below

Promoter

U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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