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Plasmid#71864Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, Isoleucine 68 mutated to AlaninePromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3C G126
Plasmid#123096PurposeExpresses 3xFLAG-LC3C G126 in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseTags3xFLAGExpressionMammalianMutationDeleted amino acids 127-147. Stop codon after G12…PromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3A G120
Plasmid#123091PurposeExpresses 3xFLAG-LC3A G120 in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
UseTags3xFLAGExpressionMammalianMutationDeleted amino acid 121. Stop codon after G120.PromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 H60A
Plasmid#71863Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, Histidine 60 mutated to AlaninePromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 L74A
Plasmid#71866Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, Leucine 74 mutated to AlaninePromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 P90A
Plasmid#71867Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, Proline 90 mutated to AlaninePromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-LC3B HRD
Plasmid#207551PurposeHomologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-LC3B
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B Q116P G120
Plasmid#129291PurposeGateway entry clone encoding human deconjugation-resistant LC3BDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseGateway entry vector / entry cloneTagsExpressionMutationChanged Glutamine 116 to Proline. Deleted amino a…PromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B G120A no-stop
Plasmid#123206PurposeGateway entry clone encoding human MAP1LC3B G120A lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseGateway entry vector / entry cloneTagsExpressionMutationGlycine 120 to Alanine, stop codon removedPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 Y71A
Plasmid#71865Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, Tyrosine 71 mutated to AlaninePromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1
Plasmid#159905PurposeMutagenesis of Slc32a1DepositorInsertSlc32a1 (Slc32a1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro mCherry-EGFP-LC3B
Plasmid#22418Purposeretroviral expression of mCherry, EGFP-tagged LC3DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (MAP1LC3B Human)
UseRetroviralTagseGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-mGFP-LC3-mRFP
Plasmid#184902PurposeStable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7, monomeric EGFP, and monomeric RFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-HT-LC3-SV40-Hygro
Plasmid#182045PurposeLentiviral expression vector encoding HaloTag-LC3 for monitoring autophagosome closureDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseLentiviralTagsHaloTag-TEVExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-mCherry-EGFP-LC3B
Plasmid#170446PurposeLentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.DepositorInsertmCherry-GFP-LC3 (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV mCherry-GFP-LC3B WT
Plasmid#123230PurposeExpresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCXI Puro DsRed-LC3-GFP
Plasmid#31182DepositorInsertDsRed-LC3-GFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQCXI Neo DsRed-LC3-GFP
Plasmid#31183DepositorInsertDsRed-LC3-GFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-LC3B G120A
Plasmid#123115PurposeExpresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFPExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-LC3B-GFP
Plasmid#123107PurposeExpresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3BDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only