Showing: 321 - 340 of 403 results
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Plasmid#61573PurposeTarget a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 geneDepositorInsertRasgrf2-2A-dCre (Rasgrf2 P1 bacteriophage, Mouse)
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-H1,NR
Plasmid#218395Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailabilityAcademic Institutions and Nonprofits only -
UPR pBAL Ura3
Plasmid#45353DepositorInsertDHFR Ub Lek Ura3
UseTagsExpressionYeastMutationPromoterpCUP1AvailabilityAcademic Institutions and Nonprofits only -
pAAH4
Plasmid#41828PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for phleomycin resistanceDepositorTypeEmpty backboneUsePcr templateTagsExpressionYeastMutationPromoterTEFAvailabilityAcademic Institutions and Nonprofits only -
pDownstream1Lox
Plasmid#164574PurposeIntegration of 1 LoxN site in the genome of P. bergheiDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…AvailabilityAcademic Institutions and Nonprofits only -
pAAH2
Plasmid#41826PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionBacterial and YeastMutationPromoterT7, TEFAvailabilityAcademic Institutions and Nonprofits only -
pAAH3
Plasmid#41827PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionYeastMutationPromoterTEFAvailabilityAcademic Institutions and Nonprofits only -
pCSJ95
Plasmid#104343PurposeExpress yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertFBP1
UseTags3xFLAGExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCSJ125
Plasmid#104344PurposeExpress yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertMDH2
UseTags3xFLAGExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGJJ001
Plasmid#183619PurposebindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHIS8-3_DL121
Plasmid#171953PurposeExpression vector for ecDHFR-asLOV2 fusion protein. Also has TYMSDepositorInsertFusion of ecDHFR and asLOV2 at position 121 of DHFR
UseTags8-His tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_DL121_WTTS
Plasmid#171954PurposeSelection vector for chimeric fusion of ecDHFR and asLOV2.DepositorInsertsFusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase
UseTags8-His tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pSL1419
Plasmid#219778PurposepDEF-TurboID-GFPmut2, negative control for cytosolic expressionDepositorInsertTurboID/GFPmut2
UsePlasmodium, rodent malaria parasitesTagsExpressionMutationPromoterPbEF1alpha promoterAvailabilityAcademic Institutions and Nonprofits only -
Calb1-T2A-dgCre targeting vector
Plasmid#114438PurposeTarget dgCre into the endogenous mouse Calb1 locus at the stop codonDepositorInsertdgCre
UseMouse TargetingTagsDHFR domain and EGFPExpressionMutationPromoternone; utilizes endogenous Calb1 promoter for expr…AvailabilityAcademic Institutions and Nonprofits only -
pAAH1
Plasmid#41825PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionBacterial and YeastMutationPromoterT7, TEFAvailabilityAcademic Institutions and Nonprofits only -
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pJO629
Plasmid#104342PurposeExpress yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertpTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
UseTags3xFLAGExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGPΩTp
Plasmid#130660PurposeInsertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.DepositorInsertΩ trimethoprim cassette
UseTagsExpressionBacterialMutationAddition of Ω sites next to trimethoprim cassette…Promoterdhfr promoterAvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-mZFP57
Plasmid#87224PurposeExpress hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coliDepositorInsertZFP57 (Zfp57 Mouse)
UseTagshisSUMOExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pGJJ045
Plasmid#183621PurposeabundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 321 - 340 of 403 results