-
Plasmid#54078PurposeLocalization: Cytoplasm, Excitation: 487, Emission: 509DepositorInsertEIF2C3 (AGO3 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSyn-Htt171-66Q-myc-WPRE
Plasmid#107934PurposeAdeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neuronsDepositorInsertExon 1 of mutant HTT, 66 polyQ repeats (HTT Human)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Htt171-18Q-myc-WPRE
Plasmid#107936PurposeAdeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neuronsDepositorInsertExon 1 of wild type HTT (HTT Human)
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-EIF2C3-N-18
Plasmid#55039PurposeLocalization: Cytoplasm, Excitation: 587, Emission: 610DepositorInsertEIF2C3 (AGO3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:NLuc-I{AGO-405}:NosT
Plasmid#212182PurposeThis binary vector expresses Nanoluciferase (Nluc) containing the Argonaut (AGO) intron (405 bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertNanoLuc luciferase-I{AGO-405}
UseTagsExpressionPlantMutationAGO intron added at bp 405, eliminates expression…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{AGO-198}:NosT
Plasmid#212169PurposeThis binary vector expresses mNeonGreen containing the Argonaut (AGO) intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{AGO-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and AGO int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-CI{AGO-198}:NosT
Plasmid#212175PurposeThis binary vector expresses mNeonGreen containing the Argonaut (AGO) intron (390 bp into the gene) with extra 3' splice site removed (corrected intron) with the truncated 35sCAMV promoter and TMV UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{AGO-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and AGO int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA Ago4
Plasmid#10824DepositorInsertArgonaute 4 (AGO4 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgo
Plasmid#210191PurposeBacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
UseTags6x His tag and SUMO tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIreSneo-FLAG/HA Ago3
Plasmid#10823DepositorInsertArgonaute 3 (AGO3 Human)
UseTagsFLAG and HAExpressionMammalianMutation1 silent mutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse eIF2C1
Plasmid#50353PurposeMouse eIF2C1 cDNA cloneDepositorInserteIF2C1 (Eif2c1 Mouse)
UseTagsExpressionBacterialMutationPromoterT3Available sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA Ago1
Plasmid#10820DepositorInsertArgonaute 1 (AGO1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA Ago2 corrected
Plasmid#10822DepositorInsertArgonaute 2 (AGO2 Human)
UseTagsFLAG and HAExpressionMammalianMutation3 silent mutations in orfPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-NHA-Ago2
Plasmid#115361PurposeExpression vector to produce NHA-tagged human Ago2 proteinDepositorInsertArgonaute 2 (AGO2 Human)
UseTagsN peptide and HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-HA-Ago2
Plasmid#115362PurposeExpression vector to produce HA-tagged human Ago2 protein without N-peptideDepositorInsertArgonaute 2 (AGO2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-3XFLAG-AGO2
Plasmid#136687PurposeExpresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)DepositorInsertargonaute RISC Catalytic Component 2 (AGO2 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-mAgo2-EGFP
Plasmid#209935PurposeLentiviral expression of mouse Argonaute-2 in mammalian cellsDepositorInsertAgo2 (Ago2 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1
Plasmid#22420DepositorInsertArgonaut-1, variant A (AGO1 Fly)
UseDrosophila expressionTagslambdaN-HAExpressionMutationPromoterAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse eIF2C2
Plasmid#50354PurposeMouse eIF2C2 cDNA cloneDepositorInserteIF2C2 (Ago2 Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA Ago2
Plasmid#10821DepositorInsertArgonaute 2 (AGO2 Human)
UseTagsFLAG and HAExpressionMammalianMutationR668H. Additionally, N terminus different from wi…PromoterAvailable sinceOct. 14, 2005AvailabilityAcademic Institutions and Nonprofits only