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Plasmid#53197PurposeExpresses HA tagged human MEK1 C121S from puromycin resistance retroviral vectorDepositorInsertMEK1 (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationC121SPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D
Plasmid#110302PurposeExpresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX302-DUSP19-V5 puro
Plasmid#87772PurposeExpresses V5 tagged human DUSP19DepositorInsertDUSP19 (DUSP19 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-GG-OCT4-1-5-PGK-Puro
Plasmid#102893PurposePiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.DepositorInsertOCT4-gRNAs-1-5-PGK-puro (POU5F1 )
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-CMYC T58A
Plasmid#53178PurposeExpresses mouse CMYC T58A from puromycin resistance retroviral vectorDepositorInsertCMYC (Myc Mouse)
UseRetroviralTagsExpressionMammalianMutationT58APromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE1-puro
Plasmid#208031PurposeEnables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-mMCL1-WT
Plasmid#45817DepositorInsertmMCL-1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-ClACE2 (Dog)
Plasmid#158083PurposeExpresses dog ACE2 in mammalian cellsDepositorInsertDog ACE2 (ACE2 )
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-MjACE2 (Pangolin)
Plasmid#158084PurposeExpresses pangolin ACE2 in mammalian cellsDepositorInsertPangolin ACE2 (ACE2 )
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmx-puro-MiGT
Plasmid#172399PurposeRetroviral expression vector for murine direct cardiac reprogrammingDepositorUseRetroviralTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-RnACE2 (Rat)
Plasmid#158086PurposeExpresses rat ACE2 in mammalian cellsDepositorInsertRat ACE2 (Ace2 Rat)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
shERK2b-mlpx-puro
Plasmid#65230Purposeencodes a shRNA against ERK2DepositorInsertshRNA against ERK2 (MAPK1 Human)
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro LIP
Plasmid#15713DepositorInsertLIP (CEBPB Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMXspuro-mCherry-NBR1
Plasmid#74242PurposeRetroviral expression of mCherry-NBR1DepositorInsertneighbor of BRCA1 gene 1 (NBR1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW-GemininΔDbox-HA-Puro
Plasmid#193770PurposeDoxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertGemininΔDbox-HA (GMNN Human)
UseLentiviralTagsHAExpressionMutationDbox removed (R23A, L26A). Codons switched at siR…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.84(hRlucCP/Puro)
Plasmid#212935PurposeRapidResponse(TM) Renilla luciferase vector for investigating regions controlling transcriptionDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Neuroligin-2 scFv [L107/90]
Plasmid#206763PurposeMammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/90 scFv.DepositorInsertNeuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBT-peRNA_GG-Puro
Plasmid#173220PurposepiggyBac transposon containing hU6 promotor and pegRNA cloning siteDepositorInsertspegRNA cloning cassette
PuroR
UsePiggybacTagsExpressionMammalianMutationPromoterEF1-a and hU6Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK
Plasmid#177868PurposeCloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).DepositorInsertmGreenLantern-based EXSISERS
UseCRISPR, Synthetic Biology, and UnspecifiedTagsOLLASExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shTGFBR3 puro
Plasmid#58696PurposeLentiviral shRNA vector for knockdown of human TGFBR3DepositorInsertshTGFBR3 (TGFBR3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-b5 ER
Plasmid#38274DepositorInsertCytochrome b5 (Cyb5a Rat)
UseRetroviralTagsEGFPExpressionMammalianMutationCyb5a Tranmembrane region from 95 to 134 amino ac…PromoterAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pirespuro2-CD86-mEos2
Plasmid#98284Purposemammalian expression of CD86-mEos2DepositorInsertCD86 (CD86 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRES-Puro-TCPTP
Plasmid#33366DepositorInsertTCPTP-45 (PTPN2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP-P2A-CIAR_pLenti_CMV_Puro
Plasmid#86503PurposeLenti plasmid for generating CIAR expressing stable cell linesDepositorInsertGFP-P2A-CIAR (SOS1 Human)
UseLentiviralTagsExpressionMammalianMutationT968L in SOScatPromoterCMVAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQHA-USP7 CS puroR
Plasmid#46754PurposeRetroviral vector that expresses catalytically inactive form of HA-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsHAExpressionMammalianMutationC223S--catalytically inactivePromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
EMSV-myogenin (puro)
Plasmid#14712DepositorInsertmyogenin (Myog Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-Puro
Plasmid#115143PurposeSafe harbor site 231 knock-in vector with puromycin resistance selection cassetteDepositorInsertPuromycin
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro SCRIB WT
Plasmid#88886PurposeSCRIB WT in MSCV backboneDepositorInsertSCRIB (SCRIB Human)
UseRetroviralTagsN/AExpressionMutationN/APromoterMSCV promoterAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-PRDX5WT-VA
Plasmid#98688PurposeLentiviral expression of human PRDX5-WT in mammalian cellsDepositorInsertPRDX5 (PRDX5 Human)
UseLentiviralTagsVA tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-sh-mFAK C
Plasmid#37015DepositorInsertsh-mFAK C (Ptk2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceMay 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
MXS_CMV::PuroR-bGHpA
Plasmid#62439PurposeMXS_chaining vector with CMV::PuroR-bGHpADepositorInsertresistance cassette against Puromycin with CMV Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAd NeuroD-I-nGFP
Plasmid#19414DepositorInsertneurogenic differentiation 1 (Neurod1 Mouse)
UseAdenoviral; Entry vectorTagsGFP and IRESExpressionMutationPromoterAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-TMPRSS11D-puro
Plasmid#158475PurposeConstitutive lentiviral expression of human TMPRSS11D.DepositorInsertTMPRSS11D (TMPRSS11D Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Neo-PuroDTK
Plasmid#84036PurposePiggyBac cassette contains Neomycin, puromycin and TK genesDepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterCAG promoterAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro-Flag-Pol b
Plasmid#23257DepositorInsertDNA polymerase beta (POLB Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
DNA-CARtcr-IRESpuro
Plasmid#89346Purpose2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassetteDepositorInsertTCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
UseLentiviralTagsSNAPf tagExpressionMammalianMutationPromoterSFFVAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro2-H2B-iRFP
Plasmid#197352PurposeEncoding nuclear-targeted iRFP713.DepositorInsertiRFP with nucleus-targeting sequence
UseLentiviralTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterEF-1αAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-NanoLuc-PuroR
Plasmid#80962PurposeCRISPaint gene taggingDepositorInsertNanoLuc
UseGene taggingTagsExpressionMutationPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (LR)-mNeonGreen-IRESpuro2
Plasmid#183743PurposeExpresses Ki67 (LR)-mNeonGreenDepositorInsertMKI67 LR domain (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationLR domain of Ki67PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNeuroguidin_AA
Plasmid#148237PurposeMammalian Expression of HsNeuroguidinDepositorInsertHsNeuroguidin (NGDN Human)
UseTagsExpressionMammalianMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-pGK:GFP
Plasmid#68486PurposeRetrovirus expressing GFP driven by pGK promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralTagsExpressionMammalianMutationPromoterLTR and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-CTSS-puro
Plasmid#158469PurposeConstitutive lentiviral expression of human CTSS.DepositorInsertCTSS (CTSS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
ME1/pBABE-puro
Plasmid#49163PurposeFor expression of Malic Enzyme 1 in mammalian cells from retroviral vectorDepositorInsertMalic Enzyme 1 (ME1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
5LTR-puro-3LTR
Plasmid#86948PurposePiggyBac transposonDepositorInsertPiggyBac transposon
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-EEA-g1-PGK-Puro
Plasmid#102908PurposePiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-guide1-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-Puro-ccdB
Plasmid#82580PurposeA modified version of plasmid pX330 (#42230): Added are a ccdB stuffer and a Puromycin selection cassetteDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCBhAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-shRFP
Plasmid#125778Purposeconstitutive expression of a short-hairpin RNA targeting RFPDepositorInsertshRFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
TNF-SBP-EGFP_puromycin
Plasmid#65284PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-CTSL-puro
Plasmid#158466PurposeConstitutive lentiviral expression of human CTSL.DepositorInsertCTSL (CTSL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_puromycin
Plasmid#65310PurposeLuminal ER hook only (RUSH system)DepositorInsertSTIM1 mutated for binding motif to microtubule (STIM1 Human)
UseTagsstreptavidinExpressionMammalianMutation2 amino acids of STIM1 (IP from SxIP motif) were …PromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only