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Plasmid#210472PurposePlasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.DepositorInsertKanMX-TetO7-CYCpr cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIG-724pUC19-tNGFR-P2A-EVER2
Plasmid#186105PurposeKnockin of truncated NGFR to target geneDepositorInsertEVER2 (TMC8 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-748pUC19-tNGFR-P2A-AP3B1
Plasmid#186106PurposeKnockin of truncated NGFR to target geneDepositorInsertAP3B1 (AP3B1 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-734pUC19-tNGFR-P2A-PIK3R1
Plasmid#186107PurposeKnockin of truncated NGFR to target geneDepositorInsertPIK3R1 (PIK3R1 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-710pUC19-tNGFR-P2A-IL10R1
Plasmid#186108PurposeKnockin of truncated NGFR to target geneDepositorInsertIL10R1 (IL10RA Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-713pUC19-tNGFR-P2A-CD45
Plasmid#186109PurposeKnockin of truncated NGFR to target geneDepositorInsertCD45 (PTPRC Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-730pUC19-tNGFR-P2A-Coronin1A
Plasmid#186110PurposeKnockin of truncated NGFR to target geneDepositorInsertCORONIN1A
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-717pUC19-tNGFR-P2A-CD3E
Plasmid#186111PurposeKnockin of truncated NGFR to target geneDepositorInsertCD3E (CD3E Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-737pUC19-tNGFR-P2A-CD18
Plasmid#186113PurposeKnockin of truncated NGFR to target geneDepositorInsertCD18 (ITGB2 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-701pUC19-tNGFR-P2A-FOXP3
Plasmid#186114PurposeKnockin of truncated NGFR to target geneDepositorInsertFOXP3 (FOXP3 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-745pUC19-tNGFR-P2A-CD4
Plasmid#186115PurposeKnockin of truncated NGFR to target geneDepositorInsertCD4 (CD4 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-721pUC19-tNGFR-P2A-RhoH
Plasmid#186116PurposeKnockin of truncated NGFR to target geneDepositorInsertRHOH (RHOH Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-744pUC19-tNGFR-P2A-CD2
Plasmid#186118PurposeKnockin of truncated NGFR to target geneDepositorInsertCD2 (CD2 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-732pUC19-tNGFR-P2A-STIM1
Plasmid#186119PurposeKnockin of truncated NGFR to target geneDepositorInsertSTIM1 (STIM1 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-720pUC19-tNGFR-P2A-ZAP70
Plasmid#186120PurposeKnockin of truncated NGFR to target geneDepositorInsertZAP70 (ZAP70 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-712pUC19-tNGFR-P2A-ITCH
Plasmid#186085PurposeKnockin of truncated NGFR to target geneDepositorInsertITCH (ITCH Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-747pUC19-tNGFR-P2A-CD7
Plasmid#186086PurposeKnockin of truncated NGFR to target geneDepositorInsertCD7 (CD7 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-735pUC19-tNGFR-P2A-PIK3CD
Plasmid#186087PurposeKnockin of truncated NGFR to target geneDepositorInsertPIK3CD (PIK3CD Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-723pUC19-tNGFR-P2A-EVER1
Plasmid#186088PurposeKnockin of truncated NGFR to target geneDepositorInsertEVER1 (TMC6 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-727pUC19-tNGFR-P2A-WASP
Plasmid#186089PurposeKnockin of truncated NGFR to target geneDepositorInsertWASP (WAS Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-716pUC19-tNGFR-P2A-CD3G
Plasmid#186090PurposeKnockin of truncated NGFR to target geneDepositorInsertCD3G (CD3G Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-714pUC19-tNGFR-P2A-SAP
Plasmid#186091PurposeKnockin of truncated NGFR to target geneDepositorInsertSAP (SH2D1A Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-731pUC19-tNGFR-P2A-MAGT1
Plasmid#186093PurposeKnockin of truncated NGFR to target geneDepositorInsertMAGT1 (MAGT1 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-728pUC19-tNGFR-P2A-WIP
Plasmid#186094PurposeKnockin of truncated NGFR to target geneDepositorInsertWIP (WIPF1 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-729pUC19-tNGFR-P2A-Moesin
Plasmid#186095PurposeKnockin of truncated NGFR to target geneDepositorInsertMOESIN (MSN Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-702pUC19-tNGFR-P2A-LRBA
Plasmid#186096PurposeKnockin of truncated NGFR to target geneDepositorInsertLRBA (LRBA Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-708pUC19-tNGFR-P2A-DOCK8
Plasmid#186097PurposeKnockin of truncated NGFR to target geneDepositorInsertDOCK8 (DOCK8 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-719pUC19-tNGFR-P2A-CD3Z
Plasmid#186098PurposeKnockin of truncated NGFR to target geneDepositorInsertCD3Z (CD247 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-742pUC19-tNGFR-P2A-Caspase8
Plasmid#186099PurposeKnockin of truncated NGFR to target geneDepositorInsertCASPASE8
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-718pUC19-tNGFR-P2A-CD3D
Plasmid#186100PurposeKnockin of truncated NGFR to target geneDepositorInsertCD3D (CD3D Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-740pUC19-tNGFR-P2A-BCL10
Plasmid#186101PurposeKnockin of truncated NGFR to target geneDepositorInsertBCL10 (BCL10 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-704pUC19-tNGFR-P2A-STAT3
Plasmid#186102PurposeKnockin of truncated NGFR to target geneDepositorInsertSTAT3 (STAT3 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-705pUC19-tNGFR-P2A-STAT5b
Plasmid#186103PurposeKnockin of truncated NGFR to target geneDepositorInsertSTAT5B (STAT5B Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
lacI-Ptac-lacO-IMBB4-pUC
Plasmid#170770Purposetest plasmid for 5RM assembly, expresses lacI repressorDepositorInsertlacI
UseSynthetic BiologyTagsExpressionMutationPromoterlacI promoterAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pUC-DEST-cjSTELLA-mCerulean-PuroTK
Plasmid#141029PurposeReporter integrationDepositorInsertmCerulean
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Prham-tagRFP-IMBB2.4-pUC57-mini
Plasmid#149343Purposeexpresses tagRFP fluorescent protein in E. coliDepositorInserttagRFP
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)
Plasmid#133791PurposeU6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018DepositorInsertSt1Cas9 sgRNA entry vector
UseTagsExpressionMammalianMutationV1 sgRNA architecture from Carter et al. biorxiv …PromoterU6Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)
Plasmid#133792PurposeU6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015DepositorInsertSt3Cas9 sgRNA entry vector
UseTagsExpressionMammalianMutationsgRNA architecture from Muller et al. Molecular T…PromoterU6Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-CD5HA2-P2A-smAID-mClover
Plasmid#110658PurposeLentiviral vector expressing cell surface HA-tag and monomeric GFP N-terminaly fused with auxin inducible degronDepositorInsertCD5HA2-P2A-AID-mClover
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Kan Level 2
Plasmid#109218PurposeNon binary level 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57 DYSF, alt exon 1, 40a
Plasmid#87894Purposeresearch of alternative isoforms of the protein dysferlinDepositorInsertDYSF, alt exon 1, 40a (DYSF Human)
UseTagsExpressionBacterialMutationalternative isoforms, see commentsPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57 DYSF, alt exon 1, 5a, 17, 40a
Plasmid#87895Purposeresearch of alternative isoforms of the protein dysferlinDepositorInsertDYSF, alt exon 1, 5a, 17, 40a (DYSF Human)
UseTagsExpressionBacterialMutationalternative isoforms, see commentsPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57 DYSF, alt exon 1a, 40a
Plasmid#87901Purposeresearch of alternative isoforms of the protein dysferlinDepositorInsertDYSF, alt exon 1a, 40a (DYSF Human)
UseTagsExpressionBacterialMutationalternative isoforms, see commentsPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57 DYSF, alt exon 1a, 17, 40a
Plasmid#87899Purposeresearch of alternative isoforms of the protein dysferlinDepositorInsertDYSF, alt exon 1a, 17, 40a (DYSF Human)
UseTagsExpressionBacterialMutationalternative isoforms, see commentsPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57 DYSF, alt exon 1, 17, 40a
Plasmid#87891Purposeresearch of alternative isoforms of the protein dysferlinDepositorInsertDYSF, alt exon 1, 17, 40a (DYSF Human)
UseTagsExpressionBacterialMutationalternative isoforms, see commentsPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 thru gap + Cys
Plasmid#49161PurposeTemplate for generating gene 60 mRNA truncated two codons after coding gap + CysDepositorInsertBacteriophage T4 gene 60
UseTagsExpressionMutationgene 60 mRNA truncated two codons after coding ga…PromoterT7Available sinceFeb. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP
Plasmid#195503PurposeSOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locusDepositorInsertSOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
UseCRISPR; Donor vectorTagsT2A-H2B-mCitrineExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only