pIG-713pUC19-tNGFR-P2A-CD45
(Plasmid
#186109)
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PurposeKnockin of truncated NGFR to target gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186109 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePUC19
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD45
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SpeciesH. sapiens (human)
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MutationWT with tNGFR sequence
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Entrez GenePTPRC (a.k.a. B220, CD45, CD45R, GP180, IMD105, L-CA, LCA, LY5, T200)
Cloning Information
- Cloning method Unknown
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.09.02.458799v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIG-713pUC19-tNGFR-P2A-CD45 was a gift from Alexander Marson (Addgene plasmid # 186109 ; http://n2t.net/addgene:186109 ; RRID:Addgene_186109) -
For your References section:
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails. Shy BR, Vykunta VS, Ha A, Talbot A, Roth TL, Nguyen DN, Pfeifer WG, Chen YY, Blaeschke F, Shifrut E, Vedova S, Mamedov MR, Chung JJ, Li H, Yu R, Wu D, Wolf J, Martin TG, Castro CE, Ye L, Esensten JH, Eyquem J, Marson A. Nat Biotechnol. 2022 Aug 25:10.1038/s41587-022-01418-8. doi: 10.1038/s41587-022-01418-8. 10.1038/s41587-022-01418-8 PubMed 36008610