-
-
ATF4 15: 5’ATF4.uORF1stopm1.GFP
Plasmid#21862DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationStop codon for uORF1 converted to UCG (see author…PromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GTSE1(638-720)-GFP
Plasmid#100735PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertGTSE1 (GTSE1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationC-terminus onlyPromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
Orai1-jGCaMP8f
Plasmid#231630PurposeGreen fluorescent reporter of Orai1-associated calcium influxDepositorInsertOrai1-jGCaMP8f (ORAI1 Human, Synthetic)
UseTagsjGCaMP8fExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
Orai1-jGCaMP8f V203Y
Plasmid#231631PurposeGreen fluorescent reporter of Orai1-associated calcium influxDepositorInsertOrai1-jGCaMP8f V203Y (ORAI1 Human, Synthetic)
UseTagsjGCaMP8f V203YExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHsp70(K71E)-GFP
Plasmid#228196Purposeexpressed human Hsp70 (K71E mutation) with a GFP fluorescent protein tagDepositorInsertHSP70(K71E) (HSPA1A Human)
UseTagsGFPExpressionMammalianMutationK71EPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN057
Plasmid#220053PurposeNMD-reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (c.3846G>A mutation; W1282X).DepositorInsertfLuc-CFTR(W1282X, exons 22-27) (CFTR Human)
UseTagsExpressionMammalianMutationc.3846G>A mutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lneg-INTR-GFP
Plasmid#208902Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius neglectus, tagged with GFPDepositorInsertINTR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
Interface-1 (b)
Plasmid#204429PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
UseTagsEGFPExpressionMammalianMutationE626A T630A K668A E671APromoterCMVAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Palpha6 and Interface-4 (a)
Plasmid#204434PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
UseTagsEGFPExpressionMammalianMutationF860A Y861A Y862A Y863A Q864A R865A H866A F867APromoterCMVAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Palpha2
Plasmid#204441PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
UseTagsEGFPExpressionMammalianMutationW771A K772A K773A R774A W775A L776A Y777A W778A K…PromoterCMVAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
nCLC-ShadowY
Plasmid#193565PurposeExpresses human Clathrin Light Chain B (neuronal isoform) tagged with ShadowY in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsShadowYExpressionMammalianMutationNeuronal isoformPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLC-FKBP-EGFP
Plasmid#193572PurposeExpresses human Clathrin Light Chain B tagged with FKBP and mEGFP in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsFKBP and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLC-ShadowY
Plasmid#193561PurposeExpresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsShadowYExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
eDrMet
Plasmid#183999PurposeOptically controlled eDrcMet expression in mammalian cellsDepositorInserteDrMet (MET Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
BtGRK2[D110A K220R]-mVenus
Plasmid#137779PurposeVisualization of GRK2DepositorInsertBovine GRK2[D110A K220R] (GRK2 Bovine)
UseTagsExpressionMammalianMutationD110A K220RPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsGRK6[L66A E514A]-sYFP2
Plasmid#137781PurposeVisualization of GRK6DepositorInsertHuman GRK6[L66A E514A] (GRK6 Human)
UseTagsExpressionMammalianMutationL66A E514APromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-HA-BACE1
Plasmid#165032PurposeHA-BACE1DepositorInsertBACE1 (BACE1 Human)
UseTagsHA (embedded after propeptide cleavage site)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDL025_HA-rb_IgG_HC_15F11
Plasmid#193622PurposeHA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.DepositorInsertAnti_HA_15F11 rabbit heavy chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL026_HA-rb_IgG_LC_15F11
Plasmid#193623PurposeHA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.DepositorInsertAnti-HA_15F11 rabbit light chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
HsB2AR-mCherry
Plasmid#137785PurposeVisualization of the Beta-2-adrenergic receptorDepositorInsertHuman beta2-adrenergic receptor (ADRB2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
VCP(wt)-EGFP
Plasmid#23971DepositorInsertValosin containing protein (VCP Human)
UseTags6xHis, EGFP, and HAExpressionMammalianMutationnonePromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
polyQ74-GFP
Plasmid#231893PurposeForms cytosolic HTT partial exon 1 Q74 aggregatesDepositorInsertHTT partial exon 1 Q74 (HTT Human)
UseTagsEGFPExpressionMammalianMutationCAG repeat expansionPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
TPST2-EGFP
Plasmid#66618PurposeEncodes GFP fusion of TPST2 trans Golgi marker (Tyrosyl protein sulfotransferase 2)DepositorInsertTPST2 (TPST2 Human)
UseTagsExpressionMammalianMutationInsert contains T370A relative to NM_001008566.1PromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
VCP(R155H)-EGFP
Plasmid#23972DepositorInsertValosin containing protein (VCP Human)
UseTags6xHis, EGFP, and HAExpressionMammalianMutationR155HPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
Cx36-GCaMP
Plasmid#123604PurposeMouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cellsDepositorInsertConnexin 36-GCaMP3 fusion (Gjd2 Mouse)
UseTagsGCaMP3ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Orai1-GCaMP6f
Plasmid#73564PurposeGreen fluorescent reporter of Orai1-associated calcium influxDepositorInsertOrai1-GCaMP6f (ORAI1 Human, Synthetic)
UseTagsGCaMP6fExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
Kif5A-CIBN
Plasmid#102251PurposeExpresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)DepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
VCP(A232E)-EGFP
Plasmid#23973DepositorInsertValosin containing protein (VCP Human)
UseTags6xHis, EGFP, and HAExpressionMammalianMutationA232EPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
-
LAMP1-SBP-GFP
Plasmid#120172PurposeExpresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)DepositorInsertLysosomal-associated membrane protein 1 (Lamp1 Rat)
UseTagsSBP and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
VCP(DK0)-EGFP
Plasmid#23974DepositorInsertValosin containing protein (VCP Human)
UseTags6xHis, EGFP, and HAExpressionMammalianMutationE305QE578QPromoterAvailable sinceMarch 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GalT-mCherry
Plasmid#214271PurposeMammalian expression of GalT-mCherry (Golgi marker)DepositorInsertGalT(1-82)-mCherry (B4GALT1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVenus-N-SLC35B2
Plasmid#155038PurposeTransient expression of SLC35B2-VenusDepositorInsertSLC35B2 (SLC35B2 Human)
UseTagsVenusExpressionMammalianMutationSee depositor comments belowPromoterCMVAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Dynamin 2 K44A
Plasmid#22301DepositorInsertDynamin 2 K44A (Dnm2 Rat)
UseTagsGFPExpressionBacterial and MammalianMutationK44APromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
PR_GEF (2XPDZ-mCherry-Larg (DH)
Plasmid#80407PurposeRecruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cellsDepositorInsert2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)
UseTagsmCherryExpressionMammalianMutationDH domain only (aa 766-997)PromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
FKBP-beta2(616-951)-GFP
Plasmid#100726PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoformPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MCS-eDHFR(69K6)-EGFP
Plasmid#172101PurposeMammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFPDepositorInsert22aa-eDHFR(69K6)-EGFP
UseTagsExpressionMammalianMutationeDHFR(69K6): Hexalysine (K6) sequence is inserted…PromoterCMVAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
psigma2-EGFP
Plasmid#53610PurposeExpresses sigma2 of adaptor protein of AP2DepositorInsertsigma2 (Ap2s1 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
HsTPC2-EYFP
Plasmid#135194PurposeExpresses tagged TPC2 in mammalian cellsDepositorInsertTPC2 (TPCN2 Human)
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta1(617-949)-GFP
Plasmid#100732PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP1B1 (AP1B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
PH-Btk(R28C)-GFP
Plasmid#51464PurposeNon-PIP3 binding control for PH-Btk-GFPDepositorInsertBTK (aa 1–177) (BTK Human)
UseTagsGFPExpressionMammalianMutationR28CPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP3-373
Plasmid#91779PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorInsertGCaMP3-373
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta3(702-1094)-GFP
Plasmid#100734PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP3B1 (AP3B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationappendage and ear onlyPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pCMV-LAMP1-mCherry
Plasmid#214274PurposeMammalian expression of LAMP1-mCherry (lysosome marker)DepositorInsertLAMP1-mCherry (Lamp1 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only
