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Showing: 801 - 850 of 14023 results

  2. Antares-SEPLuc C1A4E

    Plasmid
    #141088
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-IRESv24-SEP-C1A4E
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Please see depositor comments
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    141088-plasmid-map-sequence-id-296867

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  3. pDD2038

    Plasmid
    #101378
    Purpose
    Name: pBluescript-ΔRRE. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE.
    Depositor
    Dirk Dittmer
    Article
    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1
    Insert
    Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Blue Heron Biotechnology Inc. bidirectional mutan…
    Promoter
    Available since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    101378-plasmid-map-sequence-id-202172

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  18. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid
    #195741
    Purpose
    Base editing for making a pre-stop codon in mmoX using phenol inducible system
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Po (phoenol-inducible promoter)
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195741-plasmid-map-sequence-id-386423

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  20. pcDNA-dCas9-HA

    Plasmid
    #61355
    Purpose
    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    61355-plasmid-map-sequence-id-99781

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  30. pExpreS2-1-CR-PA-10H-SIII

    Plasmid
    #175447
    Purpose
    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Use
    Tags
    BiP Secretion Peptide and Thrombin-Protein A-10Hi…
    Expression
    Insect
    Mutation
    Promoter
    Fused Actin-HSP70
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    175447-plasmid-map-sequence-id-358139

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  36. p1585588

    Plasmid
    #62620
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    dihydroflavonol 4-reductase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62620-plasmid-map-sequence-id-103534

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  43. pTRE3G-WHG cGASdeltaN-HA

    Plasmid
    #186868
    Purpose
    Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    White-handed gibbon cGAS truncation mutant (193-557 a.a.) with C-terminal HA tag
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186868-plasmid-map-sequence-id-366570

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  44. L2096-GPA-BRET-Biosensor-Retrovirus-TD146

    Plasmid
    #222878
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    MSCV LTR
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222878-plasmid-map-sequence-id-446610

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  45. L2379-FKBP-GPA(V84R)-20-11S

    Plasmid
    #222901
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA(V84R)-20GS-NanoLuc11S
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222901-plasmid-map-sequence-id-441651

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  46. L2380-FKBP-GPA(V84R)-20-114

    Plasmid
    #222900
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA(V84R)-20GS-NanoLuc114
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222900-plasmid-map-sequence-id-441786

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  47. L2378-FRB-GPA(V84R)-20-11S

    Plasmid
    #222899
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-20GS-NanoLuc11S
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222899-plasmid-map-sequence-id-440196

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  48. L2377-FRB-GPA(V84R)-20-114

    Plasmid
    #222898
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-20GS-NanoLuc114
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222898-plasmid-map-sequence-id-440197

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  49. L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

    Plasmid
    #222875
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    MSCV LTR
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    222875-plasmid-map-sequence-id-441838

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Showing: 801 - 850 of 14023 results