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Plasmid#221407PurposeMammalian expression of human integrin beta1 N391* N562* full-lengthDepositorInsertintegrin beta1 N391* N562* full-length (ITGB1 Human)
UseTagsCD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …ExpressionMammalianMutationcodon-optimized mature sequencePromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-BTN3A2-COMP5AP-AviTag-9xHis
Plasmid#157215PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertBTN3A2 (BTN3A2 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFRSF17-COMP5AP-AviTag-9xHis
Plasmid#157053PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTNFRSF17 (TNFRSF17 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep
Plasmid#180806PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)
UseTagsExpressionBacterialMutationBTLA (Y243F, Y257F, Y282F)PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep
Plasmid#180807PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y226F, Y257F, Y282F)-TwinStrep (BTLA Human)
UseTagsExpressionBacterialMutationBTLA (Y226F, Y257F, Y282F)PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrep
Plasmid#180808PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y226F, Y243F, Y282F)-TwinStrep (BTLA Human)
UseTagsExpressionBacterialMutationBTLA (Y226F, Y243F, Y282F)PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y257F)-TwinStrep
Plasmid#180809PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y257F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y226F, Y243F, Y257F)-TwinStrep (BTLA Human)
UseTagsExpressionBacterialMutationBTLA (Y226F, Y243F, Y257F)PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-2-GST-PD-1icd (AA194-288, Y223F, Y248F)
Plasmid#180815PurposeExpression of GST fused human PD-1 intracellular domain (AA194-288, Y223F, Y248F)DepositorInsertPD-1int (AA194-288, Y223F, Y248F) (PDCD1 Human)
UseTagsExpressionBacterialMutationPD-1 (Y223F, Y248F)PromoterTacAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
ST-Tstem(5A)-GFP
Plasmid#162502PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.DepositorUseTagsGFPExpressionMammalianMutationThe mutated Tac stem region is inserted within ST…PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tac-STC(5A)
Plasmid#162491PurposeExpresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cellsDepositorInsertpartial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
UseTagsGFPExpressionMammalianMutationTac is in partial length with its stem region, TM…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
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RP2 gRNA (BRDN0001148096)
Plasmid#76282Purpose3rd generation lentiviral gRNA plasmid targeting human RP2DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
RP2 gRNA (BRDN0001145650)
Plasmid#76283Purpose3rd generation lentiviral gRNA plasmid targeting human RP2DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
RP2 gRNA (BRDN0001144983)
Plasmid#76284Purpose3rd generation lentiviral gRNA plasmid targeting human RP2DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
RP2 gRNA (BRDN0001145706)
Plasmid#76285Purpose3rd generation lentiviral gRNA plasmid targeting human RP2DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLY088_AAV_EFS-BCMA-41BBz-PRODH2
Plasmid#192191PurposeBCMA CAR AAV vector PRODH2 KI (pLY088)DepositorInsertBCMA CAR AAV vector PRODH2 KI (pLY088) (TNFRSF17 Synthetic)
UseAAV; Mammalian expressionTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral
Plasmid#207507PurposeThis plasmid can be used to generate retrovirus.DepositorInserttNGFR, HA-GD2-28z_CAR (NGFR Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0267
Plasmid#142502PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLY092b-lenti-EFS-BCMA-BBz-T2APRODH2-T2APuro-WPRE
Plasmid#192193PurposeLenti-BCMA-CAR-PRODH2DepositorInsertLenti-BCMA-CAR-PRODH2 (TNFRSF17 Synthetic)
UseLentiviral; Mammalian expressionTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_TNFRSF17_WT
Plasmid#82263PurposeGateway Donor vector containing TNFRSF17 , part of the Target Accelerator Plasmid Collection.DepositorInsertTNFRSF17 (TNFRSF17 Human)
UseGateway entry vectorTagsExpressionMutationN81S; K86EPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 IGF1R-BirA(R118G)-HA
Plasmid#109232PurposeThis construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).DepositorInsertIGF1R (IGF1R Human)
UseTagsBirA(R118G) (highly promiscuous form) and HAExpressionMammalianMutationPromoterT7Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
IGF1R_pLX307
Plasmid#98344PurposeLentiviral expression of IGF1RDepositorInsertIGF1R (IGF1R Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.NWS.VSVg_NGFR
Plasmid#158243PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHA.NWS.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ITGB1
Plasmid#51920PurposeExpresses full-length Integrin beta-1 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertITGB1 (ITGB1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationStop Codon at amino acid 728, before CD4, bio and…PromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.FLAG.VSVg_NGFR
Plasmid#158245PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHA.FLAG.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_NWS.FLAG.VSVg_NGFR
Plasmid#158233PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsNWS.FLAG.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF0256
Plasmid#142496PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_NPM1_ALK
Plasmid#205868PurposeExpress mEGFP-tagged fusion protein, NPM1_ALK from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCG_IFITM1
Plasmid#179955PurposeExpression vector for human IFITM1.DepositorInsertIFITM1 (IFITM1 Human)
UseTagsExpressionMammalianMutationP13APromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0263
Plasmid#142500PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFRSF17-Fc(DAPA)-AviTag-6xHis
Plasmid#156488PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertTNFRSF17 (TNFRSF17 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR
Plasmid#181972PurposeExpresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.DepositorInsertFMC6.3-28z CAR; LTBR (LTBR Human, Synthetic)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0260
Plasmid#142499PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PDCD1LG2_WT
Plasmid#82287PurposeGateway Donor vector containing PDCD1LG2, part of the Target Accelerator Plasmid Collection.DepositorInsertPDCD1LG2 (PDCD1LG2 Human)
UseGateway entry vectorTagsExpressionMutationF229SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
BMPR1A_pLX307
Plasmid#98320PurposeLentiviral expression of BMPR1ADepositorInsertBMPR1A (BMPR1A Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 hSCN5AdeltaSIV GFP Nter
Plasmid#145770Purposeexpresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cellsDepositorInsertsodium voltage-gated channel alpha subunit 5 (SCN5A Human)
UseTagsGFPExpressionMammalianMutationStop codon at Serine 2013PromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TEK-COMP5AP-AviTag-9xHis
Plasmid#157521PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTEK (TEK Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0264
Plasmid#142501PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0255
Plasmid#142495PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0259
Plasmid#142498PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0266
Plasmid#142578PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0268
Plasmid#142579PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0262
Plasmid#142576PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0265
Plasmid#142577PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only