Skip to main content
Addgene
Showing: 641 - 680 of 1445 results

  1. FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k

    Plasmid
    #127401
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Use
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: K295 to Amber codon, W340A,…
    Promoter
    CMV
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127401-plasmid-map-sequence-id-247963

    Enlarge

  14. MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a

    Plasmid
    #216385
    Purpose
    Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)
    Depositor
    William Weis
    Article
    Bruguera et al bioRxiv 2024.02.03.578714
    Insert
    Norrin (NDP Human)
    Use
    Baculovirus
    Tags
    GP64 signal sequence-MBP-3C and Rho1D4
    Expression
    Insect
    Mutation
    C93A/C95A/C131A
    Promoter
    Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    216385-plasmid-map-sequence-id-433265

    Enlarge

  31. C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r

    Plasmid
    #162950
    Purpose
    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    162950-plasmid-map-sequence-id-331914

    Enlarge

  32. C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

    Plasmid
    #162964
    Purpose
    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    162964-plasmid-map-sequence-id-330937

    Enlarge

  34. C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r

    Plasmid
    #162933
    Purpose
    Gateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    His6-maltose binding protein fusion, AcMNPV polyhedrin promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162933-plasmid-map-sequence-id-330541

    Enlarge

Showing: 641 - 680 of 1445 results