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pEF1a-mMLH1dn
Plasmid#174825PurposeMammalian expression of mouse MLH1dn (codon optimized)DepositorInsertmouse MLH1dn (codon optimized)
UseTagsExpressionMammalianMutationdeletion of residues 758-760PromoterEF1aAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-rIgG-Fc2-ICAM1
Plasmid#156462PurposeExpresses a rabbit Fc-ICAM1 fusion protein in mammalian cellsDepositorInsertIntercellular Adhesion Molecule 1 (Icam1 Mouse)
UseTagsICAM1-rFcExpressionBacterial and MammalianMutationendogenous signal peptide replaced with IL2 signa…PromoterhEF1-HTLCAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
p21-Reporter
Plasmid#52432PurposeDNA damage inducible Red Fluorescent Protein for mammalian expressionDepositorInsertp21-p53 Tandem Response Element Promoter
UseTagsTurboRFPExpressionMammalianMutationPromoter13X repeated p21-p53 response elementAvailable sinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-VPR-Puro
Plasmid#99373Purpose3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)DepositorInsert(Sp)dCas9-VPR-P2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hysn-flex-dsRed-shscramble
Plasmid#71383PurposeExpresses scramble shRNA Cre-dependently by flex switchDepositorInserthuman synapsin promoter-flex switch-dsRed-shscramble
UseAAV, Cre/Lox, and RNAiTagsExpressionMammalianMutationPromoterhuman synapsinAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg1
Plasmid#127158PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotypeDepositorInsertIghg1 (Ighg1 Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIgkc
Plasmid#127157PurposeExpression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant regionDepositorInsertIgkc (Igkc Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.1-mIglc2
Plasmid#127156PurposeExpression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant regionDepositorInsertIglc2 (Iglc2 Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3-myr-AKT-HA
Plasmid#31789DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
BII-CMV-AfmW3A
Plasmid#133402PurposePiggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cellsDepositorUseTagsExpressionMammalianMutationN/APromoterCMVAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato
Plasmid#60661PurposeFlp-dependent expression of Kir2.1-T2A-tdTomatoDepositorInsertFLEX- Kir2.1-T2A-tdTomato
UseAAVTagsExpressionMutationPromoterEF1αAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-N
Plasmid#37533DepositorInsertcysteine-free SGFP2-N
UseTagsExpressionMammalianMutationC48S and C70M mutations relative to SGFP2PromoterCMVAvailable sinceAug. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD1
Plasmid#20183DepositorInsertNotch 1 Intracellular Domain (Notch1 Mouse)
UseTags3XFLAGExpressionMammalianMutationContains murine Notch 1 Intracellular Domain (Val…PromoterCMVAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
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AbVec2.0-mIghg2c
Plasmid#127159PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotypeDepositorInsertIghg2c (Ighg2c Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-EGFP
Plasmid#65252Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET15b PKA Cat
Plasmid#14921DepositorInsertPKA catalytic subunit alpha (Prkaca Mouse)
UseTags6xHis and Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Grin1 KI
Plasmid#131485PurposeEndogenous tagging of GluN1: N-terminal (amino acid position: A20)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only
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