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Plasmid#22513DepositorInsertPmex-5::mCherry (w introns w/o stop)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
JH03
Plasmid#73512PurposemKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2DepositorInsertpmex-5::mKate2::PH::tbb-2
UseTagsmKate2ExpressionMutationPromotermex-5Available sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSAH71 - [AWAp | wrmScarlet | tbb-2 UTR]
Plasmid#200345PurposemScarlet BioPart for AWA neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWAp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003856, odr-10Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH72 - [AWAp | gfp | tbb-2 UTR]
Plasmid#200346Purposegfp BioPart for AWA neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWAp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003856, odr-10Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSiM4
Plasmid#199383PurposeEncodes dual cistronic membrane localized jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertjGCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA110
Plasmid#59784Purposedrives mCherry in C. elegans intestinal cellsDepositorInsertsUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJW2072
Plasmid#154334PurposeCrispr repair template: add insertion site specific homology arms by PCR before insertionDepositorInsert30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
UseCRISPRTagsExpressionWormMutationSilent mutations to remove piRNA sitesPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSP31
Plasmid#199373PurposeEncodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSP23
Plasmid#199372PurposeEncodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::GCaMP6s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEM270 - MCS mosTI hygroR target
Plasmid#159826PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertMCS mosTI hygroR target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1838
Plasmid#154324PurposesgRNA Destination Vector for SapTrapDepositorInsertSapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNM3732
Plasmid#153086Purposemec-4p GFP-C1 positive control for RMCE integrationDepositorInsertmec-4p GFP-C1 tbb-2 3'
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)
Plasmid#47387Purposeallow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectinDepositorInsertglutamate-gated chloride Channels
UseTagsExpressionMammalianMutationLeu9 prime mutated to F (Phenylalanine in M3-M4 l…PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]
Plasmid#159896PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | mNeonGreen | cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW2172
Plasmid#163096PurposeCRISPR repair template: add insertion site specific homology arms by PCR before insertionDepositorInsert30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
UseCRISPRTagsExpressionWormMutationSilent mutations to remove piRNA sitesPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1272
Plasmid#44486DepositorInsertMinimal Mos1, MCS
UseMultiple cloning site vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCM1.127
Plasmid#21384DepositorInsertpie-1 promoter (short, no ATG) in pDONRP4P1R
UseGatewayTagsExpressionMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJW6.01
Plasmid#62882PurposeTransgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTRDepositorInsertGFP::MEG-3
UseTagsGFPExpressionWormMutationPromotermeg-3Available sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1584
Plasmid#121055PurposeYPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertYPET^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized YPET, and auxi…ExpressionWormMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only