Skip to main content
Addgene
Showing: 3361 - 3380 of 17258 results
  1. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid
    #121803
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.
    Insert
    polyA + EF1a::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

    Plasmid
    #121807
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.
    Insert
    polyA + RIP::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. KO502: pMVP (L3-L2) HA tag + pA; RIP::TETa-pA

    Plasmid
    #121810
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven TETa
    Insert
    HA epitope tag-polyA + RIP::TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid
    #121815
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid
    #121818
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid
    #121820
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pMuLE ENTR U6 n.s shRNA L1-L4

    Plasmid
    #62136
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
    Depositor
    Insert
    non-silencing (scrambled) shRNA
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pMuLE ENTR U6 n.s shRNA R4-R3

    Plasmid
    #62137
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
    Depositor
    Insert
    non-silencing (scrambled) shRNA
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  9. pMuLE ENTR U6 n.s shRNA L5-L2

    Plasmid
    #62139
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
    Depositor
    Insert
    non-silencing (scrambled) shRNA
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  10. pMuLE ENTR 7SK-miR-30 R4-R3

    Plasmid
    #62121
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Type
    Empty backbone
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. pMuLE ENTR 7SK-miR-30 L1-R5

    Plasmid
    #62118
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Type
    Empty backbone
    Use
    RNAi; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  12. pNR-DEST

    Plasmid
    #90101
    Purpose
    Gateway destination vector featuring nitrate reductase (NR) promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector for protein expression…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  13. pH4-DEST

    Plasmid
    #90099
    Purpose
    Gateway destination vector featuring H4 promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector for protein expression…
    Tags
    Expression
    Mutation
    Promoter
    H4
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 3361 - 3380 of 17258 results