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Plasmid#163670PurposeExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, and TMD and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the 9 amino acids from the …PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only
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F9SF-GFP
Plasmid#163671PurposeExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from ST6GAL1 and luminal domain from TFR in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the 9 amino acids from the …PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
F9FS-GFP
Plasmid#163672PurposeExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from TFR and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the 9 amino acids from the …PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2 gRNA resistant
Plasmid#163408PurposeExpresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationgRNA-targeting sequences are substituted with nuc…PromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔEC gRNA resistant
Plasmid#163413PurposeExpresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe extracellular domain (27-150 aa) is deleted. …PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔTail gRNA resistant
Plasmid#163412PurposeExpresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe C-terminal tail domain (530-1038 aa) is delet…PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔKinase gRNA resistant
Plasmid#163411PurposeExpresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe kinase domain (202-500 aa) is deleted. gRNA-t…PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV-Blast mLST8-Flag Mut2
Plasmid#124917PurposeFor stable expression_ sgRNA resistant Flag tagging mLST8 W272F/W274F mutantDepositorInsertMLST8 (MLST8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
MOK gRNA (BRDN0001145525)
Plasmid#75936Purpose3rd generation lentiviral gRNA plasmid targeting human MOKDepositorInsertMOK (MOK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MOK gRNA (BRDN0001148493)
Plasmid#75938Purpose3rd generation lentiviral gRNA plasmid targeting human MOKDepositorInsertMOK (MOK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
JA1280
Plasmid#49969PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD HB-10 (HBB Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA1266
Plasmid#49966PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD HB-7 (HBB Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA1229
Plasmid#49955PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD HB-2 (HBB Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA1217
Plasmid#49954PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding siteDepositorInsertTet1CD HB-1 (HBB Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
p37-2iDMD-LR
Plasmid#88892Purpose37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS
Plasmid#145027PurposeLentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear markerDepositorInsert3xnls-Tq-Ca-FLITS
UseLentiviralTags3xnls-Tq-Ca-FLITS and H2B-MaroonExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DYRK1A(K188R)-2A-mCherry
Plasmid#177166PurposeExpresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Lysine at position 188 substituted with Arginine.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationChanged Lysine 188 to ArgininePromoterCMV with beta globin intronAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC38A7
Plasmid#132094PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC38A7 (SLC38A7 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223_PPP6C_WT
Plasmid#81811PurposeGateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.DepositorInsertPPP6C (PPP6C Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
MLM3739
Plasmid#49959PurposeExpresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a controlDepositorInsert3x Flag Tet1CDmut HB-4 (HBB Human)
UseTALENTags3x FLAGExpressionMutationcatalytic domain of TET1 inactive (H1671Y and D16…PromoterEF1aAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only