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Showing: 8351 - 8400 of 16262 results

  2. pFnCpf1GG

    Plasmid
    #131010
    Purpose
    Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of FnCas12a
    GFP expression cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131010-plasmid-map-sequence-id-257701

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  16. SpyTag003-OspC

    Plasmid
    #159998
    Purpose
    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells
    Depositor
    Mark Howarth
    Article
    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:
    Insert
    SpyTag003-OspC (20-209) C129S
    Use
    Tags
    His6-tag and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    C129S mutation improves expression level.
    Promoter
    T7
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159998-plasmid-map-sequence-id-316165

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  19. pcDNA3-InPAkt

    Plasmid
    #181914
    Purpose
    Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.
    Depositor
    Jin Zhang
    Article
    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10
    Insert
    InPAkt
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181914-plasmid-map-sequence-id-363357

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  32. LBR-FKBP-GFP

    Plasmid
    #222413
    Purpose
    To express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
    Depositor
    Stephen Royle
    Article
    Downie et al bioRxiv 2024.05.31.596797
    Insert
    LBR (LBR Human)
    Use
    Tags
    FKBP-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222413-plasmid-map-sequence-id-439134

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  34. pGS1222

    Plasmid
    #63791
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eGFP-Twin-Strep tag
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63791-plasmid-map-sequence-id-106478

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  39. pCRISPReporter

    Plasmid
    #65007
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65007-plasmid-map-sequence-id-109898

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  46. L2-AA007

    Plasmid
    #185750
    Purpose
    Vector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.
    Depositor
    Peter Szoevenyi
    Article
    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph
    Inserts
    HygR2
    eGFP
    Use
    Tags
    membrane localization tag Lti6b
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    185750-plasmid-map-sequence-id-367858

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  47. DT40

    Plasmid
    #80415
    Purpose
    Expresses 40 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Article
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Insert
    human DMPK exons 11-15 with 40 interrupted CTG repeats (DMPK Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80415-plasmid-map-sequence-id-151402

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Showing: 8351 - 8400 of 16262 results