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Plasmid#34571DepositorInsertArg1 promoter/enhancer -31/-3810 (Arg1 Mouse)
UseLuciferaseTagsFirefly LuciferaseExpressionMutationPromoterAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-2xSTREP-CDKN1B-Puro
Plasmid#172611PurposeRetroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cellsDepositorInsertCDKN1B (CDKN1B Human)
UseRetroviralTagsFLAG-2xSTREPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP7s
Plasmid#104463PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7s
UseTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-ER-5
Plasmid#57132PurposeLocalization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertER (CALR Human)
UseTagsmPA-GFPExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2+ GCamp6-3NLS
Plasmid#165401PurposeExpresses a nuclear localized genetically encoded calcium reporter.DepositorInsertNuclear Localization Signal
UseAvian, xenopus, zebrafishTagsGCamp6ExpressionMammalianMutationPromoterSP6Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe Blast osTIR1-9Myc
Plasmid#80073PurposeRetroviral vector for stable expression of osTIR1-9Myc in mammalian cell linesDepositorInsertOsTIR1
UseRetroviralTags9 x MycExpressionMutationPromoterGagAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
SIRT6 Flag
Plasmid#13817PurposeMammalian expression of human SIRT6 with flag tagDepositorInsertSIRT6 (SIRT6 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-KIRIN1
Plasmid#107416PurposeMammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequenceDepositorInsertKIRIN1
UseTagsNuclear exclusion sequence (NES) (LALKLAGLDIGS)ExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)
Plasmid#185910PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9=D10APromoterCMV and T7Available sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP
Plasmid#162337PurposeLentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6; mU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nv5-ER beta
Plasmid#22770DepositorInsertestrogen receptor beta (ESR2 Human)
UseTagsv5ExpressionMammalianMutationPromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
L444P GBA pcDNA3.1
Plasmid#188582PurposeEctopic expression L444P GBADepositorInsertGBA (GBA1 Human)
UseTagsExpressionMammalianMutationL444PPromoterCMVAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG RUNX3 (R122C)
Plasmid#203438PurposeMammalian expression of RUNX3DepositorInsertRUNX3 (RUNX3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
ESCRT-I
Plasmid#17634DepositorInsertUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-E988K
Plasmid#173945PurposeBacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)DepositorInsertPARP1-E988K (PARP1 Human)
UseTags6xHisExpressionBacterialMutationV762A, E988KPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-L713F
Plasmid#174794PurposeBacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationL713F, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-C125G
Plasmid#174792PurposeBacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)DepositorInsertPARP1-C125G (PARP1 Human)
UseTags6xHisExpressionBacterialMutationC125G, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
AP-SNAP25
Plasmid#167995Purposepositive selection PACE Accessory PlasmidDepositorInsertSNAP25-T7PAP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-Neo-GFP-hMyo19 iso2
Plasmid#210333PurposeOverexpression of hMyo19DepositorInserthMyo19 (MYO19 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMV306hsp
Plasmid#26155DepositorInserthsp60 promoter
UseMycobacteria integrating vectorTagsExpressionMutationPromoterAvailable sinceNov. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-hygro
Plasmid#160090PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta3(702-1094)-GFP
Plasmid#100734PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP3B1 (AP3B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationappendage and ear onlyPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti_DCLK1
Plasmid#163625PurposeExpresses DCLK1DepositorInsertDCLK1 (DCLK1 Human)
UseLentiviralTagsV5ExpressionMutationPromotersv40Available sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-RUNX3
Plasmid#139812Purposedoxycycline-inducible expression of Human RUNX3 in mammalian cellsDepositorInsertRUNX3 (RUNX3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TRBP
Plasmid#50351Purposebacterial expression of TRBPDepositorInsertTRBP (TARBP2 Human)
UseTagsHis and MycExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)
Plasmid#197511PurposeCbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacerDepositorInsertAAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
UseAAV and CRISPRTagsBPNLS and BPNLS-NpuC(intein)ExpressionMutationC-terminal mutations of SpRY(L1111R/D1135L/S1136W…PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
BtuF
Plasmid#92209PurposeExpression of BtuF which is the vitamin B12 binding protein for BtuCD ABC TransporterDepositorInsertBtuF (btuF )
UseTags6x His Tag and PelB Signal SequenceExpressionBacterialMutationBtuF signal sequence removed and uses the PelB si…PromoterT7 PromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-LKB1
Plasmid#8590DepositorInsertLKB1 (STK11 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2
Plasmid#14129DepositorInsertTSC2 (TSC2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HDAC6.DC-FLAG
Plasmid#30483DepositorInsertHDAC6 (HDAC6 Human)
UseTagsFLAGExpressionMammalianMutationCatalytically inactive mutant (H216/611A)PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-tdTomato-WPRE
Plasmid#51506PurposeCan be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoterDepositorHas ServiceAAV5InserttdTomato
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-SOX4
Plasmid#110367PurposeMammalian expression of HA-tagged SOX4DepositorInsertSOX4 (SOX4 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Fyn-SH3_5WT_S25C
Plasmid#118824PurposeBacterial expression of the SH3 domain of human FynDepositorInsertFYN (FYN Human)
UseTags6-His-TEV tagExpressionBacterialMutationS25C; See depositor comments below.PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α wt-pBabe-Puro
Plasmid#26055DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Lifeact-7
Plasmid#54663PurposeLocalization: Actin, Excitation: 604, Emission: 659DepositorTypeEmpty backboneUseTagsmCardinal-LifeactExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
p18sheLL
Plasmid#37321DepositorInsertHPV18L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Aqua
Plasmid#42889DepositorInsertAquamarine
UseTags6xHis and TEV cleavageExpressionBacterialMutationT65S and H148GPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Shh::lacZ-H11
Plasmid#139098PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterShhAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-bphC
Plasmid#128137PurposeThe cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAH2-CageCys
Plasmid#71404PurposeExpresses tRNA and synthetase for mammalian encoding of a photocaged cysteineDepositorInsertaminoacyl-tRNA synthetase for a caged cysteine
UseTagsExpressionMutationPromoterCMVAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Desmoglein2-mCherry
Plasmid#36991DepositorInsertDsg2 (DSG2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9n-EGFP-L2
Plasmid#141046PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9n-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZsGreen1-N1
Plasmid#54702PurposeLocalization: N1 Cloning Vector, Excitation: 493, Emission: 505DepositorTypeEmpty backboneUseTagsZsGreen1ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
C1A-C1A-EYFP
Plasmid#61155PurposeDiacylglycerol (DAG) reporterDepositorInsertC1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
UseTagsEYFPExpressionMammalianMutationC1A from PKCG amino acids 26-89PromoterCMVAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-PuroR [M1G]
Plasmid#171800PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgOpti
Plasmid#85681PurposeLentiviral vector to express sgRNAs. The cloning site is BsmBI.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC119-MCS
Plasmid#58807Purposecontains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pS148∙CsR
Plasmid#197858PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Ap/Cb resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6m
Plasmid#40754PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6m
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) NanoLuc
Plasmid#208576PurposeEmpty NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
Empty response element
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMammalianMutationPromoterPGK and miniPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only