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Plasmid#117714PurposeExpression of HA-tagged mVenus fused to mAIDDepositorInsertmVenus-mAID
UseTagstriple HAExpressionMammalianMutationcodon optimized mAID for murine expressionPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en1FnCas9
Plasmid#201947PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en1FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationE1369R on FnCas9PromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct_mScarlet-H_N1
Plasmid#85055PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorInsertLifeAct
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Kv2.1/RBG4
Plasmid#196947PurposePlasmid expressing an antigen targeting Kv2.1 (RBG4)DepositorInsertKv2.1 (Kcnb1 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP- SEC61B
Plasmid#121159PurposeMammalian expression of GFP tagged SEC61B, used as a general ER markerDepositorInsertSEC61B (SEC61B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7C-DNAJB6
Plasmid#177657PurposeRecombinant protein synthesis of human DNAJB6DepositorInsertDNAJB6 (DNAJB6 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-795
Plasmid#161881PurposeStandard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsMBPExpressionInsectMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-IRES-Neo
Plasmid#28019DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NR2B
Plasmid#17925DepositorInsertN-methyl D-aspartate receptor 2B (Grin2b Rat)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-MYC-t2A-BCL2
Plasmid#135306PurposeOverexpression of multiple human genes (BCL2 and MYC)DepositorUseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
beta2-spectrin-HA
Plasmid#31070DepositorInsertbeta2-spectrin (SPTBN1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pYTK083
Plasmid#65190PurposeEncodes AmpR-ColE1 as a Type 8 part to be used in the Dueber YTK systemDepositorInsertAmpR-ColE1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_2FLAG-AKAP79ΔPKA
Plasmid#183452PurposeExpression of human AKAP79 lacking the PKA binding site (Δ391-400)DepositorInsertAKAP5 (AKAP5 Human)
UseTags2xFLAGExpressionMammalianMutationaa391-400 deletedPromoterCMVAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I3R
Plasmid#125065PurposeStorage plasmid for K.marxianus right homology armDepositorInsertRight homology arm for targeting integration to SWF1/ARO1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I1R
Plasmid#125063PurposeStorage plasmid for K.marxianus right homology armDepositorInsertRight homology arm for targeting integration to LAC4
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I1L
Plasmid#125030PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to LAC4
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I3L
Plasmid#125032PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to SWF1/ARO1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5M-ADAM10
Plasmid#31717DepositorInsertADAM10 (ADAM10 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_3
Plasmid#78200PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,I76G,A197C)
UseTagsExpressionBacterialMutationA17C,I76G,A197C (please see depositor comment bel…PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRT043-CLYBL-DDdcas9-VPH
Plasmid#158091PurposeInducible expression of dCas9-VPH from the CLYBL locus for CRISPR activationDepositorInsertCLYBL-CAG-DDdCas9VPH-T2A-GFP
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF0548
Plasmid#143041PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPAX8 (PAX8 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAO122 (Apr LEU2 MRC1)
Plasmid#41923DepositorInsertMediator of the Replication Checkpoint
UseTagsExpressionMutationPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1
Plasmid#138460Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRABDepositorInsertMCP-KRAB-IRES-zsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLAIΔmls
Plasmid#24594DepositorInsertHIV-1
UseTagsExpressionMutationHuman immunodeficiency virus type 1, isolate BRU,…PromoterAvailable sinceJune 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRK5 bovine GRK5
Plasmid#14690DepositorInsertGRK5 (GRK5 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA
Plasmid#121750PurposepMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsert3x HA epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET259-pUC57 24xMS2V6
Plasmid#104391PurposeMS2V6 cloningDepositorInsert24xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mKOκ-EC18154
Plasmid#137077PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOKMS
Plasmid#51543PurposeInducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2DepositorUseLentiviralTagsExpressionMammalianMutationPromotertetOAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only