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Plasmid#158743PurposeExpresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-A (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-264 with N-linked glycosylation sites …PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-CHD5-L518A/Y619E-FRAG
Plasmid#68874PurposeIPTG inducible bacterial expression of CHD5 fragmentDepositorInsertCHD5 (CHD5 Human)
UseTagsGSTExpressionBacterialMutation331-672aa, L518A/Y619EPromotertacAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH Yap S127A
Plasmid#86497PurposeMammalian expression of FLAG-tagged Yap containing S127A mutationDepositorInsertYes associated protein 1 (YAP1 Human)
UseTagsFLAGExpressionMammalianMutationS127APromoterEF1aAvailable sinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG_FBXL7
Plasmid#171848PurposepcDNA3-FLAG_FBXL7DepositorInsertF-box and leucine rich repeat protein 7 (FBXL7 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3-EFa1-HA-Jag1
Plasmid#46051DepositorInsertJag1 (Jag1 Rat)
UseTagsHA tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 ATP5O(5’UTR)-FF
Plasmid#85486PurposeFirefly luciferase under the control of ATP5O 5'UTRDepositorInsert5'UTR of ATP5O (ATP5PO Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase
Plasmid#196185PurposeCo-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-HisDepositorInsertsUseTagsGST, HA, His6, and MBPExpressionBacterialMutationchanged Serine 9 to AlaninePromoterTacAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS
Plasmid#128833PurposeKnock-in of nuclear EYFP into mouse Elf5 locusDepositorInsertNuclear EYFP into Elf5 locus (Elf5 Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN- myc ULK1 4SA
Plasmid#27628DepositorInsertmouse myc ULK1 4SA (Myc Mouse)
UseRetroviralTagsmycExpressionMutationS467A, S555A, T574A, S637A. Initial M from ULK1 r…PromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast
Plasmid#171952PurposeAll-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMG023 MBP-Axin 384-518-His (miniAxin)
Plasmid#154012PurposeExpresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coliDepositorInsertAxin1 (AXIN1 Human)
UseTagsHis6 and MBPExpressionBacterialMutation381-518 fragmentPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN- myc ULK1 K46I
Plasmid#27627DepositorInsertmouse myc ULK1 K46I (Myc Mouse)
UseRetroviralTagsmycExpressionMutationInitial M from ULK1 removed. Silent mutation at A…PromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF086 MBP-CREB 127-135-His
Plasmid#154075PurposeExpresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coliDepositorInsertCREB1 (CREB1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 UQCC2(5’UTR)-FF
Plasmid#85487PurposeFirefly luciferase under the control of UQCC2 5'UTRDepositorInsert5'UTR of UQCC2 (C6orf125 Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-CHD5-Y619E-FRAG
Plasmid#68871PurposeMammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutationDepositorInsertCHD5 (CHD5 Human)
UseTagsFLAGExpressionMammalianMutation331-672aa, Y619EPromoterCMVAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
fox1 (a2bp1l)_R (OZ520)
Plasmid#27195DepositorInsertZinc finger array targeting fox1 (a2bp1l) (rbfox1l Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
fox1 (a2bp1l)_L (OZ519)
Plasmid#27194DepositorInsertZinc finger array targeting fox1 (a2bp1l) (rbfox1l Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Flag-SKP1-T131A
Plasmid#213734PurposeExpresses human SKP1 T131A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-T131A (SKP1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-113-117-5A
Plasmid#213643PurposeExpresses human SKP1-113-117-5A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-113-117-5A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-138-142-5A
Plasmid#213645PurposeExpresses human SKP1-138-142-5A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-138-142-5A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only