Addgene

We narrowed to 12,906 results for: flag

Showing: 3301 - 3320 of 12906 results

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pFLAG-CMV4-ZIKV-NS4B

Plasmid

#178846

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-NS4B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-AMBRA1-501*-3xFLAG

Plasmid

#174160

Purpose

PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Missing aa 501-1208

Promoter

EF1A

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-AMBRA1-217*-3xFLAG

Plasmid

#174161

Purpose

PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Missing aa 217-1208

Promoter

EF1A

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-Flag-TRAF5

Plasmid

#102679

Purpose

retroviral expression plasmid for TRAF5

Depositor

Hasam Habelhah

Article

Zhang et al J Mol Biol. 2009 Jun 12;389(3):495-510.

Insert

TRAF5 (Traf5 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-RapR-p38

Plasmid

#25935

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-p38 (Mapk14 Mouse, Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-S305E-3xFLAG

Plasmid

#69840

Purpose

This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S305E

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-FLAG S400D

Plasmid

#15940

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S400D

Promoter

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only
pCDH-puro/Flag PD-L1 N219Q

Plasmid

#121452

Purpose

expression of huamn PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N219Q

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-RhoA K6,7R

Plasmid

#11751

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

RhoA (RHOA Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

K6,7R

Promoter

Available since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pCMV 3xFLAG-ATG3 K243R

Plasmid

#129301

Purpose

Expresses pCMV 3xFLAG-ATG3 K243R in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Lysine 243 to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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myr-FLAG-MEK5-pcw107

Plasmid

#64620

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K5 (MAP2K5 Human)

Use

Lentiviral

Tags

Flag and myristoylated

Expression

Mutation

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-MTOR-D2512H

Plasmid

#69017

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-D2512H (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

change Aspartate 2512 to Histidine

Promoter

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
FTCAR2:pFlagCMV-mCAR-TVV

Plasmid

#87239

Purpose

Mammalian expression of mouse CAR, TVV isoform

Depositor

Fritz Rathjen

Insert

CXADR (Cxadr Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-Sestrin2-E451A

Plasmid

#73677

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA-DDX11-Flag-KAE

Plasmid

#120728

Purpose

Expression of a Flag-tagged version of DDX11 KAE mutant in mammalian cells

Depositor

Francesca Pisani

Article

Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

Insert

DDX11 KAE mutant (DDX11 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Substitution of E201 and Y202 with K and A

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-hINO80N2 (1-406)

Plasmid

#29442

Depositor

Ronald Conaway, Joan Conaway

Article

Chen et al J Biol Chem. 2011 Feb 8. ():.

Insert

INO80 (INO80 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

INO80 1-406 (a.a.)

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-PARL-D319A-FLAG C-t

Plasmid

#191958

Purpose

Expression of Presenilin Associated Rhomboid Like (PARL) D319A mutant

Depositor

Luca Scorrano

Insert

PARL D319A

Use

Tags

FLAG

Expression

Mammalian

Mutation

D319A

Promoter

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVneo-FYN-WT-Myc-FLAG

Plasmid

#74510

Purpose

Retroviral expression of FYN

Depositor

Lars Rönnstrand

Article

Chougule et al Oncotarget. 2016 Feb 2. doi: 10.18632/on

Insert

FYN (FYN Human)

Use

Retroviral

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

unknown

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pTT3-SP-6xHis-KIR2DL4(FL)-FLAG

Plasmid

#157624

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL4 (KIR2DL4 Human)

Use

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-Ago2-FDM5

Plasmid

#72212

Purpose

Mammalian expression of aa 518-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-517

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only