pBabe-puro-Flag-TRAF5
(Plasmid #102679)

• Purpose: retroviral expression plasmid for TRAF5
• Depositing Lab: Hasam Habelhah
• Publication: Zhang et al J Mol Biol. 2009 Jun 12;389(3):495-510. doi: 10.1016/j.jmb.2009.04.054. Epub 2009 May 3.

Backbone

• Vector backbone: pBabe-puro
• Backbone size w/o insert (bp): 5086
• Total vector size (bp): 6883
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TRAF5
• Species: M. musculus (mouse)
• Insert Size (bp): 1706
• GenBank ID: NM_011633
• Entrez Gene: Traf5
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (unknown if destroyed)
• 3′ cloning site: EcoR I (unknown if destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry