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Plasmid#162992PurposeBEAR control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENN496_miniCRISPRcharm_dSaCas9
Plasmid#220837Purposemini CRISPRcharm expression in mammalian cellsDepositorInsertmini CRISPRcharm
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-ArcΔCTD
Plasmid#119878PurposeBacterial Expression construct for the production and purification of Arc missing C-terminal DomainDepositorInsertActivity Regulated Cytoskeletal Protein (Arc Rat)
UseTagsGSTExpressionBacterialMutationDeletion of nt 832-1110PromotertacAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.GFPsm-myc.WPRE.SV40
Plasmid#98926PurposeAAV expression of GFP spaghetti monster myc constructDepositorInsertGFP-smFP-myc
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN
Plasmid#172436PurposeLentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning siteDepositorInsertsCre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIRCNpu
Plasmid#74226PurposeVector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.DepositorTypeEmpty backboneUseSynthetic BiologyTagsNpu DnaE C-intein and Npu DnaE N-inteinExpressionBacterialMutationPromoterT7-lacAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
GSK3beta  (1015)
Plasmid#49491Purposeexpresses human Gsk3betaDepositorInsertGSK3beta (GSK3B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIMAY
Plasmid#68939PurposeE. coli/staphylococcal temperature-sensitive plasmid for allelic exchangeDepositorTypeEmpty backboneUseStaphylococcal allelic exchange vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
p4489 Flag-betaTrCP
Plasmid#10865DepositorInsertbetaTrCP (BTRC Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(C163S)
Plasmid#90088PurposeExpresses caspase 3 with C163S to prevent autocatalysis in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationC163SPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Actin-7
Plasmid#56421PurposeLocalization: Actin, Excitation: 488, Emission: 507DepositorInsertActin (ACTB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro
Plasmid#213026PurposeExpresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
UseTags3xFlag (ORF2p)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-Spike
Plasmid#173921PurposeExpresses the original SARS-Cov-2 Spike Protein under a CMV PromoterDepositorInsertSARS-2 Spike Protein (S Human, Synthetic, SARS-COV-2)
UseTagsno tags, native proteinExpressionBacterial and MammalianMutationPromoterstandard CMVAvailable sinceApril 11, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF2631
Plasmid#144140PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertINSM1 (INSM1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Oct3/4-IRES-Neo
Plasmid#13461DepositorInsertOct3/4 (Pou5f1 Mouse)
UseTagsExpressionMammalianMutationP31SPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_Donor (EMM0021)
Plasmid#63934Purpose3X Flag with P2A-Neo for 3' tagging of endogenous proteinsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsGlycine-Serine-3XFlag-P2A-NeoRExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV V5-LUC Blast (w567-1)
Plasmid#21474Purpose3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, BlastDepositorInsertV5-LUC
UseLentiviral and LuciferaseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
IMPT-10314:GABBR2
Plasmid#157851PurposeHA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expressionDepositorInsertGABBR2 (GABBR2 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
CD3 delta YFP
Plasmid#11951DepositorInsertCD3delta-YFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 DNAJB2a
Plasmid#19523DepositorInsertDNAJB2a (DNAJB2 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFR
Plasmid#116731PurposeLentiviral expression of EGFRDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-Citron1
Plasmid#134303PurposeMammalian expression of direct-response citrate sensorDepositorInsertCitron1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-delSS
Plasmid#177615PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. Slippery sequence (SS) deleted (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseTagsExpressionMammalianMutationSlippery sequence deletedPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RARE-luciferase
Plasmid#13458DepositorInsertRetinoic Acid Receptor Response Element
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceNov. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV enAsCas12f-YHAM-sgRNA_v7
Plasmid#208356PurposeExpression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cellsDepositorInsertenAsCas12f-YHAM
UseTagsExpressionMammalianMutationF48Y/S188H/V232A/E316MPromoterCMVAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-mRFP-FLAG
Plasmid#34611Purpose3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tagDepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTags2xFlag and mRFPExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1-StrepII
Plasmid#174422PurposeExpresses human Drp1 Isoform 3 in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-n1-APP
Plasmid#69924Purposemammalian expression of human APP fused to EGFPDepositorInsertAPP (APP Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSGEMKv10.1
Plasmid#85704PurposeXenopus laevis oocyte expression of Kv10.1DepositorInsertKCNH1 (KCNH1 Human)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only