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Plasmid#138559PurposeExpresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSDDepositorInsertSniper-Cas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationF539S, M763I, K890NPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry
Plasmid#122132PurposeExpress a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoterDepositorInsertdCas9-10X GCN4-P2A-mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationdCas9 D10A & H840APromoterTRE3GAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_rtTA-P2A-BFP_HygroR
Plasmid#186738PurposertTA-P2A-BFP expression construct cloned into lentiviral backboneDepositorInsertrtTA
UseLentiviralTagsP2A-BFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2334
Plasmid#142036PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCTNNB1 (CTNNB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN251
Plasmid#188471PurposeLentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)DepositorInsertenCas12a-HF (gift from Dr. Keith Joung)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro
Plasmid#176850Purposelentiviral vector for doxycycline-inducible YAP1 overexpressionDepositorInserthYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationfull length YAP1PromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-puro-N-3HA-SREBP2-C-Flag
Plasmid#134287PurposeLentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2DepositorInsertSREBP2 (Srebf2 Mouse)
UseLentiviralTags3x HA and FlagExpressionMammalianMutationmouse SREBP2 precursorPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF1354
Plasmid#141817PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertILF3 (ILF3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER22-MKK6E-Flag
Plasmid#99259PurposeLentiviral vector encoding a doxycycline-inducible Flag-tagged constitutively active MKK6.DepositorInsertMKK6E (MAP2K6 Human)
UseLentiviralTagsFlagExpressionMammalianMutationS207E T211EPromoterTRE2Available sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.EFS.SpCas9.P2A.BSD
Plasmid#57821PurposeLentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-10xGCN4_Hygro
Plasmid#192651Purpose3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance markerDepositorInsertdCas9-10xGCN4
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-mCherry-lenti
Plasmid#115796PurposeExpresses mCherry in human cells, contained in a lentiviral vector.DepositorInsertmCherry
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEF-1a promoterAvailable sinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBOBI-FLuc
Plasmid#170674PurposeExpress firefly luciferase. Used in lentivirus-based SARS-CoV-2 related (MERS) pseudovirus assay.DepositorInsertluc
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMV-FAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-Oct4-EGFP
Plasmid#21319PurposeLentiviral vector, expresses EGFP under Oct3/4 promoterDepositorInsertEnhanced Green Fluorescence Protein
UseLentiviralTagsExpressionMammalianMutationPromoterOct-4 promoter regionAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB
Plasmid#122668PurposeC terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.DepositorInsertHNRNPA1 (HNRNPA1 Human)
UseLentiviralTagsmCherry-SspBExpressionMammalianMutationContains only amino acids 186-230PromoterSFFVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73022PurposeLuminescent and fluorescent dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_FliC_IRES_mCherry_PGK_tBFP
Plasmid#85438PurposeLentiviral vector for Gal4 inducible S. Typhi Flagellin with mCherry reporter and constitutive tBFP expressionDepositorInsertS. typhi FliC
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0801
Plasmid#141707PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertATF3 (ATF3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1531
Plasmid#143961PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZBTB40 (ZBTB40 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MTS-jAspSnFR3-mRuby3
Plasmid#203492PurposeMitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vectorDepositorInsertMTS-jAspSnFR3-mRuby3
UseLentiviralTagsHis-tag, Mitochondrial localization signal, and m…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only