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Plasmid#211621PurposeRhno1 T52ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationT52APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 S51A (A)-C-Myc-DDK-P2A-Puro
Plasmid#211620PurposeRhno1 S51ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationS51APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 S163A (E)-C-Myc-DDK-P2A-Puro
Plasmid#211624PurposeRhno1 S163ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationS163APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 S162A (D)-C-Myc-DDK-P2A-Puro
Plasmid#211623PurposeRhno1 S162ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationS162APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 S164A (F)-C-Myc-DDK-P2A-Puro
Plasmid#211625PurposeRhno1 S164ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationS164APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 S178A (G)-C-Myc-DDK-P2A-Puro
Plasmid#211626PurposeRhno1 S178ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationS178APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR
Plasmid#211815Purposelentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishmentDepositorInsertSpCas9, tracrRNA and puromycin selection gene
UseLentiviralTagsExpressionMammalianMutationPromoterU1a promoter, U6 promoter, and hPGK promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)
Plasmid#209086PurposeNgn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsAviTagExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
anti-NPY/Neuropeptide Y scFv [L115/13] with sortase tag
Plasmid#204426PurposeMammalian Expression of anti-Neuropeptide Y scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L115/13.DepositorInsertRecombinant mouse scFv targeting NPY/Neuropeptide Y (Homo sapiens)
UseTags6xHis tag and Sortase tagExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CCDB-N136-RsDddA-B-UGI-PURO
Plasmid#205464PurposeA backbone containing a RsDddA fragment B for constructing mitochondria-targeted RsDdCBE expression plasmidDepositorInsertMTS; N136; ccdb; RsDddA-B; UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CCDB-N136-RsDddA-D-UGI-PURO
Plasmid#205466PurposeA backbone containing a RsDddA fragment D for constructing mitochondria-targeted RsDdCBE expression plasmidDepositorInsertMTS; N136; ccdb; RsDddA-D; UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP
Plasmid#204729PurposeCre-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP
Plasmid#204727PurposeFrt-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP
Plasmid#204724PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP
Plasmid#204722PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro
Plasmid#202800PurposeLentiviral vector to express SVA-lncRNA AK057321 with ires for mcherry and puro expressionDepositorInsertSVA-lncRNA AK057321
UseLentiviralTagsExpressionMammalianMutationPromoterEFalpha (with intron)Available sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro
Plasmid#198845PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cellsDepositorInsertCOX2-Q6-R18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ATP8-W9-R15-G1333N-UGI-Puro
Plasmid#198841PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertATP8-W9-R15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro
Plasmid#198840PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertATP8-W9-L17 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro
Plasmid#198844PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cellsDepositorInsertCOX2-Q6-L18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only