Addgene

We narrowed to 15,711 results for: puromycin

Showing: 3281 - 3300 of 15711 results

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p6875 NTap-FLAG-HA-BPV1 E6

Plasmid

#37047

Depositor

Peter Howley

Article

Tan et al Proc Natl Acad Sci U S A. 2012 Apr 30.

Insert

BPV1 E6

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pX28

Plasmid

#46850

Purpose

TOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiency

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB, RFP and PGK-puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_TARBP2

Plasmid

#106119

Purpose

Lentiviral expression of TARBP2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TARBP2 (TARBP2 Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Expression

Mutation

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE

Plasmid

#101106

Purpose

Encodes FKBP-dCas9(N) fused to VEGFR2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR2-FKBP-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pT2MP.EF-Q2m

Plasmid

#129310

Purpose

QUEEN ATP sensor for 25degC, Tol2 transposon

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-2m

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PLKO.1-SMG6-CDS-1

Plasmid

#136046

Purpose

SMG6 shRNA (Targeting CDS #1) inserted into the PLKO.1 plasmid (AACTTGTAAGTAACCTGCAGC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

SMG6 (SMG6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-SMG6-CDS-2

Plasmid

#136047

Purpose

SMG6 shRNA (Targeting CDS #2) inserted into the PLKO.1 plasmid (AAGGAGTTCCAGGTGTTACTG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

SMG6 (SMG6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-Flag-mPar6a.PIG

Plasmid

#24599

Depositor

Senthil Muthuswamy

Article

Aranda et al Nat Cell Biol. 2006 Nov . 8(11):1235-45.

Insert

Par6c

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
SEPLuc

Plasmid

#141102

Purpose

bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1α

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-CNPshRNA2

Plasmid

#126612

Purpose

Expresses shRNA against human CNP under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

CNP ShRNA-2

Use

RNAi

Tags

Expression

Mutation

Promoter

Mouse U6 promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLenCMV-Mars1CyeGFP

Plasmid

#75224

Purpose

Lentiviral transfer vector encoding Mars1Cy-eGFP fusion for intracellular expression

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

Mars1Cy-eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ1666

Plasmid

#51485

Purpose

miniMos transposable element with PuroR, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pSuperRetro-puro-RalB 45i

Plasmid

#11122

Depositor

Christopher Counter

Article

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalAB siRNA (RALA, RALB Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
MSCV-Flag-mPar6aK19A

Plasmid

#24598

Depositor

Senthil Muthuswamy

Article

Aranda et al Nat Cell Biol. 2006 Nov . 8(11):1235-45.

Insert

Par6c

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19A

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pGALT-EGFP-IRES-puro2b

Plasmid

#26463

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

pGalT

Use

Tags

EGFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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px459-Rheb sgRNA

Plasmid

#133768

Purpose

Expresses Cas9 and human Rheb sgRNA

Depositor

Shawn Ferguson

Article

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

Rheb sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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LV-TRE-p65 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60627

Purpose

Expresses p65 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

p65 mouse MyoD

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pS2-FLAG-Sestrin2-E451A

Plasmid

#73679

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

Sesn2

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5-hygro-eGFP-HEC1-8A/S55D

Plasmid

#114032

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

Hec1 - 8A/S55D

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV Cyclin D2-long-mut

Plasmid

#21657

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

mutated miR-15/16 and let-7 binding sites

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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