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pMulti

Plasmid

#228613

Purpose

Mammalian expression of a single fluorescent protein and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSU19mCherry

Plasmid

#225181

Purpose

Medium copy cloning vector with mCherry integrated in the multiple cloning site. There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAM315 pACYC-His6-Rpn10[ΔUIM]

Plasmid

#226348

Purpose

Expression of yeast Rpn10 with the UIM deleted

Depositor

Andreas Martin

Article

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

rpn10 (RPN10 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

delta UIM

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2142 MTK3-sfGFP

Plasmid

#226661

Purpose

MTK3 part containing the coding sequence of sfGFP

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2620 MTK3-TetR

Plasmid

#226670

Purpose

MTK3 part containing the coding sequence of TetR

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

TetR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3445 MTK3-mTagBFP2

Plasmid

#226662

Purpose

MTK3 part containing the coding sequence of mTagBFP2

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

mTagBFP2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2621 MTK3-LacI

Plasmid

#226671

Purpose

MTK3 part containing the coding sequence of LacI

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

LacI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM256

Plasmid

#226900

Purpose

mEOS3.2-GS-SUMO-1(3-103)-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Use

Tags

GS-SUMO-1(3-103)-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM255

Plasmid

#226899

Purpose

mEOS3.2-GS-MBP-10xN-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Use

Tags

mEOS3.2-GS-MBP-10xN-Pex15(1-309)-Prescission-GSS-…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM249

Plasmid

#226893

Purpose

Pex15(43-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Use

Tags

prescission -GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Pex15(aa 43-309)

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM034_P7_3"Lys3

Plasmid

#216459

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''lys3

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA6c_PsulA_sfGFP

Plasmid

#224587

Purpose

SOS response reporter

Depositor

Ophelia Venturelli

Article

Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PsulA

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pTE5400_pMBP_CDS dropout vector

Plasmid

#221189

Purpose

Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

Depositor

Tobias Erb

Article

Barthel et al Synthetic Biology, 2024;, ysae013

Type

Empty backbone

Use

Tags

10x His-tag + E. coli maltose binding protein (MB…

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT030

Plasmid

#225176

Purpose

Low copy cloning vector with mCherry integrated in the multiple cloning site (Chlr). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2483 (pSpy1C)

Plasmid

#220248

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM040_P8b_5'Ura4

Plasmid

#216461

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'ura4

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM036_P7_3"Ura4

Plasmid

#216465

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''ura4

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM024_P2_pMPA1

Plasmid

#216449

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter mpa1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM022_P2_pRPS1002

Plasmid

#216447

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rps1002

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM020_P2_pTRS1

Plasmid

#216445

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter trs1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge