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CLTA-mCherry HDRT Source (pTR 177)

Plasmid

#112017

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-mCherry HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-HA-DLX2-T2A-mCherry

Plasmid

#178585

Purpose

Cre-dependent expression of HA-DLX2 and mCherry under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

DLX2 (DLX2 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Dnmt3a2-Ires2-mCherry-SV40

Plasmid

#186969

Purpose

PiggyBac vector encoding mouse Dnmt3a2 with Ires2-mCherry fluorescent marker for expression in mammalian cells.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

Dnmt3a2 (Dnmt3a Synthetic, Mouse)

Use

Piggybac

Tags

Flag

Expression

Mammalian

Mutation

Isoform 2 (residues 220–908)

Promoter

CAG

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-His-TEV-mCherry-OPTN WT

Plasmid

#190191

Purpose

Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

optineurin (OPTN Human)

Use

Tags

6xHis, TEV, and mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE

Plasmid

#174134

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Expression

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p403 – TDH3-MTS-mCherry-GFP1-10-His

Plasmid

#177730

Purpose

mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

mCherry-GFP1-10

Use

Tags

Mitochondrial Targeting Sequence

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHBS1551 H14-SUMO-TDP43 F-S-TEV-mCherry

Plasmid

#133321

Purpose

Bacterial expression of TDP43 with F-S mutant CTD

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Bacterial

Mutation

All F in the TDP43 IDRs mutated to S

Promoter

Available since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(+7A)-IDR-mCherry-CRY2-NLS

Plasmid

#145261

Purpose

Mammalian Expression of Nuclear Opto HOXD13(+7A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+7A)-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeat expanded

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-SacB-Cas9-T2A-mCherry_(BbsI)

Plasmid

#117070

Purpose

Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

Depositor

Kim Failor

Inserts

Cas9
SacB

Use

CRISPR

Tags

3x FLAG, NLS, and T2A-mCherry

Expression

Mammalian

Mutation

Promoter

CBh and U6

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-p2A-LIC1 Y101A, Y103A

Plasmid

#74605

Purpose

expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

Y101A, Y103A

Promoter

SFFV

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-Cb5-IRES-puromycin-pLVx-EF1a

Plasmid

#177434

Purpose

To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

Depositor

David Andrews

Insert

Cb5 tail anchor (Cyb5r4 Rat)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

Plasmid

#112496

Purpose

Expresses channel rhodopsin in astrocytes

Depositor

Baljit Khakh

Article

Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

Insert

ChR2(H134R)

Use

AAV

Tags

mCherry

Expression

Mutation

H134R

Promoter

GfaABC1D

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-NEUROD1-T2A-mCherry

Plasmid

#178584

Purpose

Cre-dependent expression of NEUROD1 and mCherry under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

NEUROD1 (NEUROD1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)

Plasmid

#191151

Purpose

Expression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1L72:EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0313 SFFV-mCherry-SG(s70587)SG-Cre (FLP-IN)

Plasmid

#191149

Purpose

Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, Cre output derived from a gift from prof. Liqun Luo

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:Cre

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-DIO-mCherry-nega miR

Plasmid

#137732

Purpose

Lentivirus vector for control miR in a Cre-dependent manner

Depositor

Tomoyuki Furuyashiki

Article

Nie et al Neuron. 2018 Aug 8;99(3):464-479.e7. doi

Insert

control microRNA

Use

Lentiviral

Tags

mChherry

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-DIO-mCherry-TLR4miR-TLR2miR

Plasmid

#137733

Purpose

Lentivirus vector for TLR2/4 miR in a Cre-dependent manner

Depositor

Tomoyuki Furuyashiki

Article

Nie et al Neuron. 2018 Aug 8;99(3):464-479.e7. doi

Insert

TKR2/4 microRNA

Use

Lentiviral

Tags

mChherry

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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hSyn ArcLightCo (Q239)-T2A-nls-mCherry

Plasmid

#85844

Purpose

Genetically encoded voltage sensor ArcLight- codon optimized

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

ArcLightCo-Q239

Use

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

hSyn1

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4

Plasmid

#123658

Purpose

DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.

Depositor

Stanley Qi

Article

Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)

Plasmid

#191175

Purpose

Mammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP (frameshifted)

Use

Tags

Expression

Mammalian

Mutation

EGFP is frameshifted

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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