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Plasmid#216438PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter eno101
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-HR1bR181A R185A-GFP-L (B3-B4)
Plasmid#211857PurposeMutant E3-DART degron casette for N-terminal fusion to protein of interest using GoldenbraidDepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionMutationR181A R185APromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)
Plasmid#211861PurposeNuclear-localized E3-DART ligase in domestication vector.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HAExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)
Plasmid#211863PurposeNuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HAExpressionMutationC492APromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdO2c
Plasmid#210231Purposemedium-copy plasmid for bacterial expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdO1c
Plasmid#210230Purposehigh-copy plasmid for bacterial expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
NBla_1.2_GpA_G83I
Plasmid#207844PurposeBLaTM-System GpA G83I negative control in NBLa 1.2 vectorDepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
NBla_1.2_EmrE_TMD4_3P_G90V_G97V
Plasmid#207847PurposeBLaTM-System; Antiparallel negativecontrol EmrE_TMD4_3P_G90V_G97V in NBLa 1.2 vectorDepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
NBla_1.2_EmrE_TMD4_3P
Plasmid#207846PurposeBLaTM-System; Antiparallel positive control EmrE_TMD4_3P in NBLa 1.2 vectorDepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM493
Plasmid#140191PurposeORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.DepositorInsertTEV-Flag-Gly4-eGFP
UseTagsFlag + eGFPExpressionBacterialMutationPromoterPGroELAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
NBla_1.2_GpA_wt
Plasmid#207842PurposeBLaTM-System GpA wt positive control in NBLa 1.2 vectorDepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG-BC-TurboID-GOI
Plasmid#209390PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG-GOI-DE-TurboID
Plasmid#209388PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB273
Plasmid#203612PurposeTerminator of pyr4 gene from T. reesei.DepositorInsertTpyr4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB413
Plasmid#203617PurposeCoding sequence for phleomycin resistance.DepositorInsertble
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB414
Plasmid#203618PurposeCoding sequence for terbinafine resistance.DepositorInsertergA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB416
Plasmid#203620PurposeTerminator of acetamidase-encoding gene in A. niger.DepositorInsertTamdS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB389
Plasmid#203624PurposePromoter of the highly-expressed pyruvate kinase gene from A. niger.DepositorInsertPpkiA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB405
Plasmid#203626PurposePromoter of the glucoamylase gene from A. niger, maltose/starch-inducible.DepositorInsertPglaA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB271
Plasmid#203610PurposePromoter of pyr4 gene from T. reesei.DepositorInsertPpyr4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only