Addgene

We narrowed to 34,768 results for: kan

Showing: 3261 - 3280 of 34768 results

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pBCiG2

Plasmid

#149470

Purpose

Broad-Host range expression vector, pBBR origin, CinR(AM) activator, PCin promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinR(AM)-GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PCin

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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RepX_pET28a(+)

Plasmid

#172199

Purpose

Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

Depositor

Taekjip Ha

Article

Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

Insert

RepX superhelicase

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

C18L, C43S, C167V, S400C, C612A

Promoter

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDD131

Plasmid

#119886

Purpose

Optimized aTc inducible pTet promoter for Rhodococcus opacus expressing mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

Insert

pTet_mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSCAC-69

Plasmid

#31241

Purpose

Mitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafish

Depositor

Seok-Yong Choi

Article

Kim et al Biotechniques. 2008 Sep . 45(3):331-4.

Insert

mitochondrial localization enhanced green fluorescent protein

Use

Tags

Expression

Mutation

Promoter

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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slc1a3b:mCD8mCherry

Plasmid

#170207

Purpose

Drives mCD8mCherry expression with the slc1a3b promoter in astrocytes

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

mCD8

Use

Zebrafish

Tags

mCherry

Expression

Mutation

Promoter

slc1a3b

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDD87

Plasmid

#119892

Purpose

The pLPD03031 promoter from Rhodococcus opacus PD630 expressing GFP+ on the pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

Insert

pLPD03031_GFP+

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pFF745

Plasmid

#61450

Purpose

IPTG-inducible SCRIBE_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_kanR(ON)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-FKBP-nTEVP

Plasmid

#179675

Purpose

ER-associated FKBP split nTEVP (Rapalog inducible)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

FKBP-nTEVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRGD-KmR

Plasmid

#74108

Purpose

Plasmid containing kanamycin resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

aphII

Use

Bacterial vector based on pmb1 replicon

Tags

Expression

Mutation

Promoter

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Orai3

Plasmid

#16370

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai3 (ORAI3 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
YFP-GID1

Plasmid

#37305

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

See comments

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only
pKESK22

Plasmid

#64857

Purpose

expression vector, low to medium copy number, tac promoter lacI, kanamycin resistance, p15A origin of replication

Depositor

Karin Schnetz

Article

Stratmann et al J Bacteriol. 2008 Feb;190(3):926-35. Epu

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW AKAR3-EV

Plasmid

#182533

Purpose

Expresses the PKA FRET sensor AKAR3-EV in budding yeast

Depositor

Bas Teusink

Article

Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

Insert

AKAR3-EV

Use

Tags

Expression

Yeast

Mutation

Promoter

PMA1

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-KRAS-G12V

Plasmid

#179654

Purpose

KRAS active mutant (G12V mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS G12V

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-KRAS-G12D

Plasmid

#179656

Purpose

KRAS active mutant (G12D mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS G12D

Use

Tags

Expression

Mammalian

Mutation

G12D

Promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDR-GW AT1.03YEMK

Plasmid

#28004

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03YEMK

Use

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

M60Y, G131E, K132M, 133K

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pET11b-HA2

Plasmid

#165992

Purpose

Influenza HA ministem with removable HisTag

Depositor

Vesa Hytönen

Article

Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

Insert

HA2

Use

Tags

Foldon and HisTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTO-KRAS-G12C

Plasmid

#179655

Purpose

KRAS active mutant (G12C mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS G12C

Use

Tags

Expression

Mammalian

Mutation

G12C

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA12DKR-J

Plasmid

#58306

Purpose

GeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Insert

Genomic cassette

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pRGM1

Plasmid

#48789

Purpose

HA-I-SceI expression plasmid, episomal

Depositor

Michael Glickman

Article

Gupta et al Mol Microbiol. 2011 Jan;79(2):316-30. do

Insert

I-SceI

Use

Tags

HA tag

Expression

Bacterial

Mutation

Promoter

MOP

Available since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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