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Plasmid#120559PurposeExpresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells & for virus productionDepositorInsertHRAS (HRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE
Plasmid#101101PurposeEncodes dCas9(N) fused to transmembrane tetherDepositorInsertTMt-dCas9(N)
UseCRISPR and Synthetic BiologyTagsHA and MycExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro
Plasmid#120564PurposeExpresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationQ61KPromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase
Plasmid#188391PurposeReporter for BCAM expressionDepositorInsertBCAM reporter
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro
Plasmid#177201Purposeencoding a dominant negative form of CEBP under inducible promoterDepositorArticleInsertdnCEBP (A-C/EBP)
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI
Plasmid#188703PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-puro-BGHpA-TRE-pEF-tagRFP-T-NLS
Plasmid#163083PurposeDonor vector for integration of TagRFP-T reporter at AAVS1 safe harbor site via TALENsDepositorInsertTagRFP-T
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2
Plasmid#99694PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Mouse, Synthetic)
UseAAV and CRISPRTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE
Plasmid#101108PurposeEncodes dCas9(N) fused to BDKRB2DepositorInsertBDKRB2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro
Plasmid#120563PurposeExpresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer
Plasmid#86195PurposeU6 based expression of N meningitidis sgRNADepositorInsertnmCas9 sgRNA cloning cassete
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE
Plasmid#101114PurposeEncodes dCas9(N) fused to CXCR4DepositorInsertCXCR4-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAVPR2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE
Plasmid#101112PurposeEncodes dCas9(N) fused to AVPR2DepositorInsertAVPR2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE
Plasmid#101106PurposeEncodes FKBP-dCas9(N) fused to VEGFR2DepositorInsertVEGFR2-FKBP-dCas9(N)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE
Plasmid#101116PurposeEncodes dCas9(N) fused to LPAR1DepositorInsertLPAR1-dCas9(N)
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-Mid/pIRES puro Glue (Q513-E784)
Plasmid#73455PurposeExpress human middle part (Q513-E784) Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsCBP tag, HA tag, and strep tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro
Plasmid#205631PurposeExpression of TnpBmax in mammalian cellsDepositorInsertISDra2-TnpB optimized for mammalian expression
UseTagsNLS-3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)
Plasmid#225756PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Squash-FTL-IRE
Plasmid#216828PurposeExpresses a circular RNA containing a Squash aptamer and an IRE (FTL derived) in mammalian cells, using the Tornado expression system.DepositorInsertTornado-Squash-FTL-IRE
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Squash-TfR-IRE
Plasmid#216829PurposeExpresses a circular RNA containing a Squash aptamer and an IRE (TfR derived) in mammalian cells, using the Tornado expression system.DepositorInsertTornado-Squash-TfR-IRE
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only