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Plasmid#79745PurposeThis plasmid encodes the kinase domain of KCC1G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC1G
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KCC2A_HUMAN_D0
Plasmid#79746PurposeThis plasmid encodes the kinase domain of KCC2A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC2A
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1G2_HUMAN_D0
Plasmid#79716PurposeThis plasmid encodes the kinase domain of KC1G2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKC1G2
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MK03_HUMAN_D0
Plasmid#79688PurposeThis plasmid encodes the kinase domain of MK03. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK03
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAK4_HUMAN_D0
Plasmid#79698PurposeThis plasmid encodes the kinase domain of PAK4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPAK4 (PAK4 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAK6_HUMAN_D0
Plasmid#79703PurposeThis plasmid encodes the kinase domain of PAK6. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPAK6 (PAK6 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNC2
Plasmid#72438PurposeProduces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPro64 to Arg; Asp65 to His; Gln206 to ArgPromoterT7 and n/aAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK516
Plasmid#71578PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges glutamate-435 to glutaminePromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK524
Plasmid#71580PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges asparagine-347 to alaninePromoterT7Available sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK513
Plasmid#71576PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges glutamate-294 to alaninePromoterT7Available sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK564
Plasmid#71581PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges serine-71 to alaninePromoterT7Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK517
Plasmid#71579PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges glutamate-435 to aspartatePromoterT7Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK514
Plasmid#71577PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges glutamine-435 to alaninePromoterT7Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK512
Plasmid#71575PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationchanges arginine-228 to glutamatePromoterT7Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3H09
Plasmid#71003PurposeGateway entry cloneDepositorInsertSNF4Agamma (SNF4Agamma Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3C07
Plasmid#70985PurposeGateway entry cloneDepositorInsert14-3-3zeta (14-3-3zeta Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2B09
Plasmid#70918PurposeGateway entry cloneDepositorInsertgro (gro Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3C03
Plasmid#70959PurposeGateway entry cloneDepositorInsertTaf4 (Taf4 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL5B
Plasmid#67326PurposehArl5b with stop codon in Gateway Entry vectorDepositorInsertARL5B (ARL5B Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S,NoCys)
Plasmid#66877PurposeExpresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1
UseTagsHistagExpressionBacterialMutationfour (all) cysteines mutated to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only