Addgene

We narrowed to 4,919 results for: pcDNA3.1+

Showing: 3241 - 3260 of 4919 results

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pmyc-GFP-TNRC6A-(935-1026)-NES-mut

Plasmid

#42021

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa935-1026, F951A, F955A, I958A, F960A

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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mc2 mCherry

Plasmid

#206219

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-PH

Plasmid

#96948

Purpose

EGFP-PLC pleckstrin homology domain for CALI validation

Depositor

Marcel Bruchez

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

Pleckstrin homology domain

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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Venus wGBD

Plasmid

#85222

Purpose

Venus tagged Cdc-42 sensor

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Insert

wGBD (WASL Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Cdc42 binding domain of N-WASP

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-2xFLAG-Atoh1

Plasmid

#101734

Purpose

Expresses mouse Atoh1 with FLAG tag

Depositor

Albert Edge

Article

Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2x FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-optoB2-SS-YFP

Plasmid

#75270

Purpose

Arrestin independent version of optoB2 with serine mutations at S362A,S363G

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

Arrestin independent version of optoB2

Use

Tags

eGFP

Expression

Mammalian

Mutation

serine mutations at S362A,S363G

Promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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untargeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45215

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Aurora B FRET sensor

Use

Tags

CyPET, YPET

Expression

Mammalian

Mutation

Promoter

t7

Available since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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BLING 1

Plasmid

#171647

Purpose

Expresses bioluminescent glutamate indicator in mammalian cells

Depositor

Assaf Gilad

Article

Petersen et al ACS Synth Biol. 2023 Jul 14. doi: 10.102

Insert

BLING 1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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HiBiT-NbV5

Plasmid

#201490

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

HiBiT-NbV5

Use

Tags

HiBit

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pNIR-FbGFPY58/C-PKI

Plasmid

#184685

Purpose

NIR-Fb-based PKA inhibitor

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-FbGFPY58/C-PKI

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pNLRP11major.MYC

Plasmid

#131196

Purpose

Mammalian expression of human NLRP11 188S

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188S (NLRP11 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

188S

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFH2.4_mCherry-MMLV_Psi

Plasmid

#205522

Purpose

Express mCherry-Psi (export tag consisting of MMLV Psi)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-NBid-PhoCl1-CBid

Plasmid

#164050

Purpose

Optogenetic manipulation of cell apoptosis using PhoCl1

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NBid-PhoCl1-CBid

Use

Tags

kozak sequence

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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mc2 neo

Plasmid

#206214

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FRB-VMAc-CGFP

Plasmid

#70226

Purpose

Expresses C-terminal portion of GFP along with VMA intein for protein splicing

Depositor

Perry Howard

Article

Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

Insert

eGFP

Use

Tags

FKBP-rapamycin binding (FRB) domain of the mammal…

Expression

Mammalian

Mutation

Changed Ile 129 to Cys, Deleted amino acids 1-128

Promoter

CMV

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3 SWS cone opsin

Plasmid

#109363

Purpose

Human SWS cone opsin with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

SWS cone opsin (OPN1SW Human)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTS2410-Tier1-PhCMV-Myr_mTagBFP2

Plasmid

#169535

Purpose

Tier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDN41

Plasmid

#61344

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

Depositor

Roland Eils, Barbara Di Ventura

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Use

Tags

GS linker-AsLov2-biNLS9 and PKIt NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 SV8

Plasmid

#184011

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Wild type cDNA

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pVH72-Tier1-PhCMV-SEAP

Plasmid

#169560

Purpose

Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven human secreted placental alkaline phosphatase

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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