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Plasmid#53151Purposeexpression vector for catalytically inactive human Sirtuin7DepositorInsertSirt7 (SIRT7 Human)
UseTagsFLAGExpressionMammalianMutationH187Y, catalytic inactivePromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT:IL2_SigP:NLuc
Plasmid#197266PurposeDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.DepositorInsertInterleukin-2 homology arms
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
B2M Bulldozer (gRNA crB2M_13)
Plasmid#84381PurposeThis plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cellsDepositorInsertB2M Bulldozer crB2M_13 gRNA
UseTagsExpressionMammalianMutationPromoterhU6 promoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0100
Plasmid#141514PurposeLentiviral vector for overexpressing the NOV transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNOV (CCN3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-NanoLuc-BRAF-CAT
Plasmid#194772PurposeMammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.DepositorInsertBRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)
UseTagsNanoLucExpressionMammalianMutationPromoterCMV51Available sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-BRAF-REG-Halo
Plasmid#194771PurposeMammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.DepositorInsertBRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMV51Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT_5'-xrRNA_NLuc_3'-XAP1
Plasmid#197264PurposeCloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.DepositorTypeEmpty backboneUseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT:NEAT1-v2_SigP:NLuc
Plasmid#197268PurposeDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.DepositorInsertNEAT1-v2 homology arms
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
1D3 HC
Plasmid#170670PurposeMammalian Expression Plasmid of Anti-cytochrome c, 1D3 HC (Human)DepositorInsertHC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
1D3 LC
Plasmid#170669PurposeMammalian Expression Plasmid of Anti-cytochrome c, 1D3 LC (Human)DepositorInsertLC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
RCAS-Neu HA SIINFEKL
Plasmid#125832PurposeRetroviral expression of rat Neu cDNADepositorInsertNeu (Erbb2 Rat)
UseRetroviralTags2x HA and SIINFEKLExpressionMammalianMutationVal to Glu point mutation of codon 664 and trunca…PromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT:NEAT1-total_SigP:NLuc
Plasmid#197267PurposeDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.DepositorInsertNEAT1-total homology arms
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT_SigP:NLuc
Plasmid#197263PurposeCloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.DepositorTypeEmpty backboneUseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMDLg/pRRE
Plasmid#12251Purpose3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)DepositorInsertHIV-1 GAG/POL
UseLentiviral; PackagingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSV-Rev
Plasmid#12253Purpose3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)DepositorInsertRev (rev )
UseLentiviral; PackagingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112924PurposepiggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7A
Plasmid#28047DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and EGFPExpressionMammalianMutationPromoterAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
p2,0_3E5E_eGFP
Plasmid#69359PurposeEncodes for Ebola Minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoterDepositorInsert3E5E eGFP minigenome
UseT7TagsExpressionMutationPromoterT7Available sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-bActinCDS-bActinUTR-MS2V5
Plasmid#102718PurposeHalo-Actin reporter for detection of both mRNA and proteinDepositorInsertHaloTag-bActinCDS-bActin3'UTR-MS2V5
UseLentiviralTagsHaloTag and MS2V5ExpressionMutationPromoterUbCAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7A T22N
Plasmid#28048DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and EGFPExpressionMammalianMutationT22NPromoterAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only