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pHYDuet183
Plasmid#41685DepositorInsertPhoRadA intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
MLM3636
Plasmid#43860Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoterDepositorInsertNone
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pStA212
Plasmid#114171PurposeStart-Stop Assembly Level 212 plasmidDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-HIF1B [F446L] (#931)
Plasmid#99917Purposemammalian expressionDepositorInsertHIF1B (ARNT Human)
UseTagsFLAGExpressionMammalianMutationF446LPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-SOX9
Plasmid#172302PurposeIn vitro transcription of SOX9DepositorInsertSOX9 (SOX9 Human)
UseOtherTagsExpressionMutationPromoterT7 promoter A-initiatingAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
mApple-CENPB-N-22
Plasmid#54877PurposeLocalization: Nucleus/Centromeres, Excitation: 568, Emission: 592DepositorInsertCENPB (CENPB Human)
UseTagsmAppleExpressionMammalianMutationaa 1-169 of CENPB (DNA Binding Domain)PromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet26
Plasmid#41689DepositorInsertTvo VMA intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep128
Plasmid#136872PurposeMammalian expression of the centrosomal protein Cep128 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep128 (CEP128 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-hMLH1dn
Plasmid#222997PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPE7-P2A-hMLH1dn
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDeletion of residues 754-756 in MLH1 as described…PromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA4-RFP
Plasmid#62250Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
UseTagsExpressionBacterialMutationPromoterpA4Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
cre_CD
Plasmid#66030PurposeMoClo Basic Part: CDS - cre recombinase (recombines DNA at loxP sites) [C:cre:D]DepositorInsertRecombinase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAN-OR
Plasmid#62307Purposesynthetic circuit: OR gateDepositorInsertsA2NT sgRNA
A4NT sgRNA
A2NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterPA2, PPhlF, and pBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
Plasmid#221081PurposeSEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Tc
Plasmid#222336Purposeflp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3DepositorUseTagsExpressionMutationPromotercl, flp, and sacBAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Km
Plasmid#222334Purposeflp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3DepositorUseTagsExpressionMutationPromotercl, flp, and sacBAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
UseTagsMaltose binding protein and His tagExpressionBacterialMutationPromoterVhbAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-TAF15
Plasmid#181713PurposeGalactose inducible expression of TAF15DepositorInsertTAF15 (TAF15 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-pVhb-tdTER-fdh
Plasmid#215649PurposepBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoterDepositorInsertstrans-enoyl-CoA reductase
formate dehydrogenase
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only
