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pRetroSuper-shYes

Plasmid

#26987

Depositor

Joan Massague

Article

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

shRNA Yes (YES1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pCDH-CRY2-mCh-superPLDx30

Plasmid

#188988

Purpose

A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Use

Tags

CRY2 and mCherry

Expression

Mammalian

Mutation

K109R, P245A, G328S, G381V, G429D

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDSAP

Plasmid

#62293

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe HAII RLF CAAX

Plasmid

#12592

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

RLF-CAAX (Rgl2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
3486 pBABE Bid p22 GFP

Plasmid

#8777

Depositor

Stanley Korsmeyer

Article

Zha et al Science 2000 Dec 1;290(5497):1761-5.

Insert

Bid p22 (Bid Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pMIR-D10 UTR

Plasmid

#19117

Depositor

Bob Weinberg

Article

Ma et al Nature. 2007 Oct 11. 449(7163):682-8.

Insert

HOXD10 3' UTR (HOXD10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
p663-UBC-miniTurbo-V5-DSTYK _IDG-K

Plasmid

#158182

Purpose

Gateway destination clone of DSTYK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-DSTYK (DSTYK Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-Sox10

Plasmid

#64890

Purpose

Retroviral expression of murine Sox10

Depositor

Ivana de la Serna

Article

Marathe et al PLoS One. 2013 Jul 16;8(7):e69037. doi:

Insert

sox10 (Sox10 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
MSCV-19a-20-19b

Plasmid

#24827

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

mir19a-mir20-mir19b

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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hTRAF2K31RpLPCX-HA-Flag/P876

Plasmid

#20231

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K31R (a92g in the TRAF2 CDS sequence).

Promoter

Available since

March 3, 2009

Availability

Academic Institutions and Nonprofits only
pLJM1-FLAG-Sestrin2-E451A

Plasmid

#73677

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pT2P-EF-Q7mu

Plasmid

#129303

Purpose

Positive control for ATP measurement, mitochondria outer membrane, Tol2 transposon

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-7mu

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDREV-0

Plasmid

#26750

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Insert

H2B-Venus CDS (H2BC3 Human)

Use

Cre/Lox; Flp/frt; dre/rox

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pX330(puro) Rosa26-H3F3B

Plasmid

#73131

Purpose

Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

Depositor

Agnel Sfeir

Article

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

spCas9

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shMOCS3_2

Plasmid

#102974

Purpose

Suppression of MOCS3

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shMOCS3_2 (MOCS3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shMOCS3_1

Plasmid

#102973

Purpose

Suppression of MOCS3

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shMOCS3_1 (MOCS3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgACO1_1

Plasmid

#169885

Purpose

Disrupt ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgACO1_2

Plasmid

#169892

Purpose

Disrput ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF2-3'UTR

Plasmid

#136041

Purpose

UPF2 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CGCGAGGGTTAATCTTCTCTT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF2 (UPF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM sh1 DNMT1

Plasmid

#88884

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.1 DNMT1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV (spleen focus forming virus)

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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