-
Plasmid#181972PurposeExpresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.DepositorInsertFMC6.3-28z CAR; LTBR (LTBR Human, Synthetic)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin
Plasmid#99277PurposeDual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistanceDepositorInsertsH2B-mOrange2-IRES-EGFP
Puro
UseLentiviralTagsEGFP, H2B, IRES, PGK, and mOrange2ExpressionMutationPromoterEF1 alpha-human and PGK-mouseAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro
Plasmid#83107Purpose3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptideDepositorUseLentiviralTags2A self cleaving peptideExpressionMammalianMutationR24C and p64 isoformPromoterhPGK and hPGK (single transcript)Available sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR
Plasmid#181973PurposeExpresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.DepositorInsertFMC6.3-BBz CAR; LTBR (LTBR Human, Synthetic)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CENP-B targeted Aurora B FRET sensor Kif2 substrate
Plasmid#45214DepositorInsertCENP-B targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationPromotert7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)
Plasmid#214880PurposeLentiviral vector encoding RfxCas13d targeting CD46 guideDepositorInserthU6-crCD46-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR
Plasmid#181975PurposeExpresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.DepositorInsertFMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
UseLentiviralTagsExpressionMutationNGFR truncation: aa 11-276PromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG536)
Plasmid#139443PurposeLentiviral vector to ectopically express human NCOA7 isoform 4 from SFFV promoterDepositorInsertFLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro beta-catenin P2A eGFP-NLS
Plasmid#155189PurposePiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFPDepositorInsertbeta-catenin (Ctnnb1 Mouse)
UseUnspecified; Piggybac transposonTagsP2A eGFP NLSExpressionMammalianMutationSites phosphorylated by GSK-3beta have been mutat…PromoterTRE3GAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-p62 D337, 338, 339A
Plasmid#38280DepositorInsertSequestosome-1 (Sqstm1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationchanged D337A, D338A, D339APromoterAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR
Plasmid#171963PurposeDonor plasmid for CLTX T-CAR expression in human cellsDepositorInsertSynthetic CLTX CD32a-tm CD3z CAR
UseCRISPR, Synthetic Biology, and TALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor
Plasmid#12153DepositorInsertRhoA Biosensor (RHOA Human)
UseRetroviralTags6XHisExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro
Plasmid#120560PurposeExpresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus productionDepositorInsertHRAS (HRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationG12VPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)
Plasmid#214877PurposeLentiviral vector encoding RfxCas13d targeting LPT guide arrayDepositorInserthU6-crLPT-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro
Plasmid#170467PurposeThe plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistanceDepositorArticleInsertNucleocapsid
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEF1Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXD023 - pLKO-EF1a-Puro-P2A-FLuc-WPRE
Plasmid#192203PurposeLenti-PL(Puro-Luciferase)DepositorInsertLenti-PL(Puro-Luciferase)
UseLentiviral; Mammalian expressionTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG-CLTX CD32a ITAM CAR
Plasmid#171964PurposeDonor plasmid for CLTX CD32a-ITAM CAR expression in human cellsDepositorInsertSynthetic CLTX CD32a ITAM CAR
UseCRISPR, Synthetic Biology, and TALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin
Plasmid#99278PurposeDual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistanceDepositorInsertsH2B-mVenus-IRES-mCherry
Puro
UseLentiviralTagsH2B, IRES, PGK, mCherry, and mVenusExpressionMutationPromoterEF1 alpha-human and PGK-mouseAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR
Plasmid#207805PurposeE-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.DepositorInsertE-NoMi
UseLentiviralTags3xFlag tag, copGFP, mCherry, and nanoluciferaseExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171596PurposeEctodomain deleted negative control ACE2 from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only