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Plasmid#164633PurposeExpresses JIP4 in mammalian cells with a 2xmyc tagDepositorInsertJIP4 (SPAG9 Human)
UseTags2xmycExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-Med25
Plasmid#64771PurposeMammalian protein expression of flag-tagged Med25DepositorInsertMed25 (MED25 Human)
UseTagsFlagExpressionMammalianMutationPromoterpCMVAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB6
Plasmid#63107PurposeExpression of HSPB6 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb6 (HSPB6 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
mMib1-FLAG
Plasmid#37116DepositorInsertMib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1)) (Mib1 Mouse)
UseTags3 x FLAGExpressionMammalianMutationACC Kozak sequence added before ATG annotated.PromoterCMVAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV14/p14ARF
Plasmid#78760PurposeTo overexpress p14ARF in Mammalian CellsDepositorInsertARF (CDKN2A Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-ABEmax N-terminal
Plasmid#137173PurposeExpresses the N-terminal split-intein fragment of ABEmax from the CMV promoterDepositorInsertNpu-ABEmax_N-terminal
UseTagsExpressionMammalianMutationCas9 D10APromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB4
Plasmid#63105PurposeExpression of HSPB4 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb4 (CRYAA Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-BE3.9max N-terminal
Plasmid#137171PurposeExpresses the N-terminal split-intein fragment of BE3.9max from the CMV promoterDepositorInsertNpu-BE3.9max_N-terminal
UseTagsExpressionMammalianMutationCas9 D10APromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-s-pep
Plasmid#47438PurposeFRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
UseTags10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…ExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
SECURE BE4max(R33A) (pBM608)
Plasmid#140251PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFPDepositorInsertBE4max(R33A)
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, D10A in Cas9PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
CGBE1-VRQR (pBM1192)
Plasmid#140254PurposeCMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFPDepositorInserteUNG-BE4max(R33A)_VRQR-ΔUGI
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-Jun4A-Myc
Plasmid#47444Purposemammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)DepositorInsertJun 4A mutant (Jun Mouse)
UseTagsFlag and mycExpressionMammalianMutationfour N-terminal residues phosphorylated by JNK, s…PromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
AID'-BE4max
Plasmid#174696PurposeBase EditingDepositorInsertAID'-BE4max (AICDA Human)
UseTagsExpressionMammalianMutationUGI-E11KPromoterCMVAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-saBE3.9max C-terminal
Plasmid#137180PurposeExpresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoterDepositorInsertNpu-saBE3.9max_C-terminal
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-saBE3.9max N-terminal
Plasmid#137179PurposeExpresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoterDepositorInsertNpu-saBE3.9max_N-terminal
UseTagsExpressionMammalianMutationCas9 D10APromoterCMVAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG
Plasmid#122475Purposeexpresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codonPromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
2xmyc-RAB35
Plasmid#164632PurposeExpresses RAB35 in mammalian cells with a 2xmyc tagDepositorInsertRAB35 (RAB35 Human)
UseTags2xmycExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPACE-ΔUGI (pRZ1836)
Plasmid#140243PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFPDepositorInsertSPACE-ΔUGI
UseTagsExpressionMammalianMutationV82G in TadA*, D10A in Cas9, R187W in pmCDA1PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9 PE2*-3HA
Plasmid#169854PurposeMammalian Expression, SpyCas9 PE2* prime editor with 3HA-tagDepositorInsertSpCas9-H840A-star-M-MLV-3HA
UseTags3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…ExpressionMammalianMutationPromotercmvAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
NLS-nCas9-NLS-P2A-EGFP (pRZ70)
Plasmid#123616PurposeCMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).DepositorInsertNLS-nCas9-NLS-P2A-EGFP
UseTagsExpressionMammalianMutationD10A in SpCas9PromoterCMVAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only