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pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

Plasmid

#172620

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry

Plasmid

#35502

Purpose

AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

C128S and D156A

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

Plasmid

#131508

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUBC

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry-t2a-SOX2-p2a-KLF4

Plasmid

#193296

Purpose

episomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry-t2a-SOX2-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179117

Purpose

Expresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Fermt2 sgRNA

Use

AAV

Tags

Expression

Mutation

Promoter

U6

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)

Plasmid

#86416

Purpose

ONM/ER split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP11-mCherry-Scs2TM

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP-SFPQ-IRES-mCherry

Plasmid

#166950

Purpose

Lentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ (SFPQ Human)

Use

Lentiviral

Tags

GFP and mCherry

Expression

Mutation

Promoter

eF1a

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-P(Per2)-DIO-mCherry-Venus-NLS-D2

Plasmid

#110058

Purpose

Per2 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and mCherry and Venus (Per2 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Mutation

Promoter

Per2

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

Plasmid

#84604

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

8xGliBS-delta-crystallin minimal promoter

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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CAG FLEX BARK D110A p2A mCherry

Plasmid

#117694

Purpose

CAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

Cre/Lox

Tags

HA / FLAG

Expression

Mutation

D110A

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pPB UbC Arrestin-uTEV2-V5; UAS-mCherry

Plasmid

#173123

Purpose

Expresses uTEV2 and reporter receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-uTEV2-V5; UAS-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

Plasmid

#172627

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM235 - [Pmlc-1 | mcherry | cbr-tbb-2 UTR]

Plasmid

#159900

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | mcherry | cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Pmlc-1

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry

Plasmid

#139649

Purpose

Reporter assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

H2B-mCherry

Use

Lentiviral

Tags

FLAG, His6

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-ChR2-mCherry

Plasmid

#137144

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

H134R

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-rM3D(Gs)-mCherry-WPREpA

Plasmid

#154869

Purpose

Human synapsin-1 promoter; Flp-dependent rM3D(Gs) DREADD-mCherry

Depositor

Ulrik Gether

Insert

rM3D(Gs)-mCherry

Use

AAV; Flp-frt

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Human Synapsin-1

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-PonA-BI-Gl-GFP-PTC-mCherry-WT (5FP-WP)

Plasmid

#189795

Purpose

Express GFP/mCherry linked wild-type and PTC-containing beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cells

Depositor

Robert Singer

Article

Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

Insert

beta-globin (HBB Human, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ponasterone A inducible

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

Plasmid

#87828

Purpose

High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Article

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

NLS
PTS1

Use

Tags

EGFP and mCherry

Expression

Mammalian

Mutation

Promoter

CMV promoter, tetracycline operator

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)

Plasmid

#86417

Purpose

luminal split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-mCherry-SCS2TM-GFP11

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry

Plasmid

#114322

Purpose

Mifepristone-inducible lentiviral backbone for Gateway cloning of Gene insert together with constitutive mCherry expression

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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