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Showing: 8001 - 8050 of 148051 results

  1. pHN767

    Plasmid
    #178884
    Purpose
    Expression of bacterial PNGase
    Depositor
    Kazuhiro Abe
    Insert
    Peptide:N-glycosidase F
    Use
    Tags
    GST, His8
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178884-plasmid-map-sequence-id-354970

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  3. T7 promoter + terminators reporter plasmid

    Plasmid
    #156456
    Purpose
    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.
    Depositor
    Matthew D. Shoulders
    Article
    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560
    Inserts
    NeoR/KanR
    TcR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ATG start codon mutated to ACG
    Promoter
    Em7 and tet
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156456-plasmid-map-sequence-id-311167

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  4. pCAGGS-ROSAf

    Plasmid
    #54323
    Purpose
    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid
    Depositor
    Silvestro Conticello
    Insert
    TALEN for the murine ROSA26 locus
    Use
    Mouse Targeting and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGG
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    54323-plasmid-map-sequence-id-124428

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  6. pUC19-TSV (linearized in LT)

    Plasmid
    #70033
    Purpose
    pUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding region
    Depositor
    Mariet Feltkamp
    Article
    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.
    Insert
    TSPyV full genome, linearized in Large T-antigen
    Use
    Cloning vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    70033-plasmid-map-sequence-id-129217

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  16. TetO-dCas9-mD3A

    Plasmid
    #78255
    Purpose
    Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter
    Depositor
    Grant Challen
    Article
    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.
    Insert
    DNMT3A CD aa 598-912 (DNMT3A Human)
    Use
    CRISPR
    Tags
    Flag epitope tag
    Expression
    Mammalian
    Mutation
    E756A
    Promoter
    CMV
    Available since
    June 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    78255-plasmid-map-sequence-id-150192

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  17. pCAGGS-ROSAr

    Plasmid
    #54324
    Purpose
    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid
    Depositor
    Silvestro Conticello
    Insert
    TALEN for the murine ROSA26 locus
    Use
    Mouse Targeting and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGG
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    54324-plasmid-map-sequence-id-124429

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  20. pTH385-GST-TEV-SUP35

    Plasmid
    #29744
    Depositor
    Tobias von der Haar
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    wild type
    Promoter
    Available since
    Aug. 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    29744-plasmid-map-sequence-id-13828

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  21. 2xFKBP-mCh-KDEL

    Plasmid
    #173010
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    2xFKBP-mCh-KDEL
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173010-plasmid-map-sequence-id-339203

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  30. dFP-mini

    Plasmid
    #120543
    Purpose
    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.
    Depositor
    Brian Chow
    Article
    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/
    Insert
    de novo fluorescence-activating protein mini (dFP-mini)
    Use
    Tags
    6xHis - TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    120543-plasmid-map-sequence-id-234163

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  34. pOpen-Exonuclease III E. coli

    Plasmid
    #165571
    Purpose
    Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    Exonuclease III E. coli
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165571-plasmid-map-sequence-id-340929

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  35. pAAV-GFAP-EGFP

    Plasmid
    #50473
    Purpose
    EGFP under the control of GFAP promoter
    Depositor
    Bryan Roth
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Expression
    Mutation
    Promoter
    GFAP
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50473-plasmid-map-sequence-id-182310

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  36. pJR101

    Plasmid
    #187241
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Jonathan Weissman, Marco Jost
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    187241-plasmid-map-sequence-id-374769

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  44. Lamp1-mTurquoise2

    Plasmid
    #98828
    Purpose
    In vivo visualization of lysosomes (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Lamp1
    Use
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98828-plasmid-map-sequence-id-190174

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  46. pExp-PpiB

    Plasmid
    #112572
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-PpiB and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112572-plasmid-map-sequence-id-217222

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  49. pCAGGS_3E5E_eGFP

    Plasmid
    #103054
    Purpose
    Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter
    Depositor
    Elke Mühlberger
    Article
    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do
    Insert
    Ebola virus (ebolavirus Zaire, Mayinga) minigenome
    Use
    Tags
    3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    103054-plasmid-map-sequence-id-197128

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Showing: 8001 - 8050 of 148051 results