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CGBE1-NG (pBM1246)

Plasmid

#140256

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_NG-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB5

Plasmid

#63106

Purpose

Expression of HSPB5 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb5 (CRYAB Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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CGBE1 (pRZ3885)

Plasmid

#140252

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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12xMoonTag-12xSunTag-kif18b-24xPP7

Plasmid

#128606

Purpose

Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

12xMoonTag-12xSunTag
kinesin family member 18b (KIF18B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pIRIGF-IKZF3

Plasmid

#69046

Purpose

expression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

IRES

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB7

Plasmid

#63108

Purpose

Expression of HSPB7 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb7 (HSPB7 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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A3G-BE5.14

Plasmid

#163638

Purpose

C to T DNA base editing

Depositor

Xue Gao

Article

Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

Insert

A3G-BE5.14

Use

Tags

BPSV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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A3G-BE5.13

Plasmid

#163637

Purpose

C to T DNA base editing

Depositor

Xue Gao

Article

Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

Insert

A3G-BE5.13

Use

Tags

BPSV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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A3G-BE4.4

Plasmid

#163636

Purpose

C to T DNA base editing

Depositor

Xue Gao

Article

Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

Insert

A3G-BE4.4

Use

Tags

BPSV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pMGF196

Plasmid

#97005

Purpose

Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

LEMD2 (LEMD2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV delta 5

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB8

Plasmid

#63109

Purpose

Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb8 (HSPB8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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miniCGBE1-NG (pBM1182)

Plasmid

#140257

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)_NG-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Frt-hspB5

Plasmid

#63096

Purpose

Expression of HSPB5 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb5 (CRYAB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCS6-ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB

Plasmid

#111504

Purpose

Expresses a zinc finger specific to the human VEGFA locus, which is linked to a KRAB transcriptional repression domain via a StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].

Depositor

Michael Lin

Article

Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

Insert

ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB

Use

Tags

Expression

Mammalian

Mutation

The HCV NS3 protease carries F43L, Q80K, S122R, a…

Promoter

CMV

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA_B2AR-i-pep

Plasmid

#47436

Purpose

FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Article

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Use

Tags

10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
IL2R/hE-cadherin-cytotail

Plasmid

#45773

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

IL2R/hE-cadherin-cytotail (IL2RA, CDH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Interleukin-2 receptor α subunit extracellular an…

Promoter

CMV

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pS6-hα2M-H6

Plasmid

#128495

Purpose

Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (G27-S1468) (A2M Human)

Use

Tags

His tag (6x)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l4-G'-IRES-EmGFP

Plasmid

#209127

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G'-IRES-EmGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l4-G-IRES-EmGFP

Plasmid

#209126

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G-IRES-EmGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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LLP773_pLenti-6xHRBKET-sgRNA

Plasmid

#211766

Purpose

Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

pCMV and U6

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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