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pCMV-EPN-03 (G2A)

Plasmid

#80104

Purpose

Myr2(G2A)-12GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-03 (G2A)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-07 (2AA3)

Plasmid

#80105

Purpose

Palm1(2AA3)-12GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-07 (2AA3)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-08 (2AA3)

Plasmid

#80106

Purpose

Palm2(2AA3)-12GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-08 (2AA3)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN18 (R40A)

Plasmid

#80107

Purpose

PH(R40A)-4GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-18 (R40A)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01(ΔI)

Plasmid

#80048

Purpose

Expression of EPN-01 assembly mutant L33R

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(L33R)

Use

Tags

Myc

Expression

Mammalian

Mutation

L33R

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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9E10.ChS78&81A.Gal4.pCDNA1

Plasmid

#21901

Depositor

David Ron

Article

Wang et al Science. 1996 May 31. 272(5266):1347-9.

Insert

CHOP (Ddit3 Mouse)

Use

Tags

GAL4-DNA-BD and Myc

Expression

Mammalian

Mutation

S78A S81A

Promoter

Available since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pOsTIR1w/oGFP

Plasmid

#102883

Purpose

Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

OsTIR1

Use

Tags

3x myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-sfGFP 150TAG

Plasmid

#85483

Purpose

Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

superfolder GFP 150TAG

Use

Tags

Expression

Bacterial

Mutation

150TAG (amino acid 150 mutated to stop codon)

Promoter

Arabinose

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCTCON2 Aga2P-HRP

Plasmid

#73152

Purpose

Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Aga2P-HRP

Use

Tags

HA tag and myc tag

Expression

Yeast

Mutation

Promoter

GAL1

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Use

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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TurboID-mNeongreen-3xFLAG fusion pos3

Plasmid

#172864

Purpose

C. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion protein

Depositor

Mario de Bono

Article

Artan et al J Biol Chem. 2021 Sep;297(3):101094. doi

Insert

TurboID

Use

Unspecified

Tags

FLAG, mNeongreen, and myc

Expression

Mutation

Promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA flag PPAR gamma

Plasmid

#8895

Purpose

Mammalian expression of flag-tagged PPAR gamma

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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PB_TRE_Z18-PDGFR_EF1a_eGFP

Plasmid

#186304

Purpose

Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

Z18-PDGFR
eGFP

Use

Tags

HA, myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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NbAlfa-GFP2

Plasmid

#201485

Purpose

BRET2 detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbAlfa-GFP2

Use

Tags

GFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMA3790

Plasmid

#70110

Purpose

mtDNA elimination/production of rho-0 cells

Depositor

Mikhail Alexeyev

Article

Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

Inserts

EGFP
hUNG1 Y147A
Neo

Use

Tags

None, human OTC mitochondrial targeting sequence,…

Expression

Mammalian

Mutation

Y147A, aa's 1-77 removed

Promoter

CMV, EF1a, and SV40 and native

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRcCMV Cep215 (Nigg CW493)

Plasmid

#41152

Depositor

Erich Nigg

Article

Graser et al J Cell Sci. 2007 Dec 15;120(Pt 24):4321-

Insert

Cep215 (CDK5RAP2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCTcon2-5INS_16

Plasmid

#102292

Purpose

Yeast Surface Display of 5Ins_16 scFV

Depositor

Sarel Fleishman

Article

Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

Insert

Insulin binding scFV

Use

Tags

3x(G4S) Linker, G3S Linker, HA, and Myc

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCTcon2-5INS_EV3

Plasmid

#102293

Purpose

Yeast Surface Display of 5Ins_16_EV3

Depositor

Sarel Fleishman

Article

Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

Insert

Evolved Insulin binding scFV

Use

Tags

3x(G4S) Linker, G3S Linker, HA, and Myc

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiRhoA2G

Plasmid

#40179

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

RhoA Biosensor

Use

Lentiviral

Tags

His-Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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