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Plasmid#156413Purposekdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sitesDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseZebrafish transgenesisTagsEYFPExpressionMutationPromoterkdrlAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 743 (pDONR221-EYFP-hKRAS4B-WT)
Plasmid#156402PurposeGateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codonDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseGateway cloningTagsEYFPExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV-STAT31F
Plasmid#134725PurposeExpresses chimeric STAT3-STAT1 protein with human STAT3 amino acids 1-514 joined to human STAT1 at amino acid 509, with a FLAG epitope tag at the C-terminus. Based on addgene plasmid #8691 and #8707DepositorUseTagsFLAG and human STAT3 aa 1-514 joined to human STA…ExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV-STAT13F
Plasmid#134723PurposeExpresses chimeric STAT1-STAT3 protein with human STAT1 amino acids 1-508 joined to human STAT3 at amino acid 515, with a FLAG epitope tag at the C-terminus. Based on addgene plasmids #8691 and #8707DepositorUseTagsFLAG and human STAT1 aa1-508 joined to human STAT…ExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S12TAG
Plasmid#107155PurposeMBP-rPKL construct for production and purification of rat PKL pS12DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 12 to TAG for phosphoserine insertionPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S113TAG
Plasmid#107157PurposeMBP-rPKL S113TAG for production and purification of rat PKL pS113DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 113 changed to TAG for phosphoserine inser…PromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) TSPAN9
Plasmid#107502PurposeExpresses TSPAN9, puromycin resistantDepositorInsertTSPAN9 (TSPAN9 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ARSI_WT
Plasmid#81898PurposeGateway Donor vector containing ARSI , part of the Target Accelerator Plasmid Collection.DepositorInsertARSI (ARSI Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HAPLN1_p.S326N
Plasmid#81640PurposeGateway Donor vector containing HAPLN1, part of the Target Accelerator Plasmid Collection.DepositorInsertHAPLN1 (HAPLN1 Human)
UseGateway entry vectorTagsExpressionMutationS326NPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HAPLN1_p.K115N
Plasmid#81646PurposeGateway Donor vector containing HAPLN1, part of the Target Accelerator Plasmid Collection.DepositorInsertHAPLN1 (HAPLN1 Human)
UseGateway entry vectorTagsExpressionMutationK115NPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HAPLN1_p.P233H
Plasmid#81648PurposeGateway Donor vector containing HAPLN1, part of the Target Accelerator Plasmid Collection.DepositorInsertHAPLN1 (HAPLN1 Human)
UseGateway entry vectorTagsExpressionMutationP233HPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ARSI_p.P228L
Plasmid#81569PurposeGateway Donor vector containing ARSI ,part of the Target Accelerator Plasmid Collection.DepositorInsertARSI (ARSI Human)
UseGateway entry vectorTagsExpressionMutationP228LPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ARSI_p.V373M
Plasmid#81481PurposeGateway Donor vector containing ARSI , part of the Target Accelerator Plasmid Collection.DepositorInsertARSI (ARSI Human)
UseGateway entry vectorTagsExpressionMutationV373MPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
CASK gRNA (BRDN0001149157)
Plasmid#77401Purpose3rd generation lentiviral gRNA plasmid targeting human CASKDepositorInsertCASK (CASK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CASK gRNA (BRDN0001145000)
Plasmid#77402Purpose3rd generation lentiviral gRNA plasmid targeting human CASKDepositorInsertCASK (CASK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CASK gRNA (BRDN0001148068)
Plasmid#77403Purpose3rd generation lentiviral gRNA plasmid targeting human CASKDepositorInsertCASK (CASK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 4A
Plasmid#59907PurposeExpresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationmutated four serines to alaninesPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSWHhc-Lam
Plasmid#133902PurposeNegative control when used in combination with pAWH-largeT. Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWHDepositorInsertLMNA (Lam) (LMNA Human)
UseTagsExpressionYeastMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
PB-tetO-hNIL
Plasmid#197089PurposePiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiationDepositorUseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Lamin-A
Plasmid#206027PurposeExpresses GFP-tagged Lamin A in mammalian cellsDepositorInsertLamin-A (LMNA Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only