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pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

Plasmid

#174891

Purpose

amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PVT1-Tol2

Plasmid

#113381

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Tol2 reporter

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
pCMV-CRY2(D387A)-mCherry

Plasmid

#58369

Purpose

Light insensitive CRY2 mutant

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (D387A) (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

D387A

Promoter

CMV

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pQCXIP TBRII

Plasmid

#19147

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

Transforming growth factor, beta receptor II (TGFBR2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
His-Keap1-Tev-Halo

Plasmid

#87545

Purpose

expresses C-terminal Halo-tagged human Keap1 in mammalian cells

Depositor

Yimon Aye

Article

Parvez et al Nat Protoc. 2016 Dec;11(12):2328-2356. d

Insert

Keap1 (KEAP1 Human)

Use

Tags

Halo and His-Tag

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pKD077

Plasmid

#136473

Purpose

SSB-mTur2 IDL fusion expression plasmid

Depositor

James Keck

Article

Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

Insert

SSB-mTur2 (ssb E. coli)

Use

Tags

mTurquoise2

Expression

Bacterial

Mutation

mTurquoise2 inserted between Phe148 and Ser149

Promoter

T7 promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK3b(Glu)

Plasmid

#50449

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 218 to Glutamic Acid, changed Thre…

Promoter

CMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
pMIY-mETV3-Flag

Plasmid

#34579

Depositor

Peter Murray

Article

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Use

Retroviral

Tags

FLAG and IRES-YFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
MTK8b_004

Plasmid

#123892

Purpose

Encodes the 3rd gen. 5' HIV LTR cassette as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

5' HIV LTR (3rd gen.)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB3045(gRNA XI-3)

Plasmid

#73287

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2+PhyBΔCreC

Plasmid

#131781

Purpose

Mammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC

Use

Cre/Lox; Zebrafish expression

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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MTK7_014

Plasmid

#123881

Purpose

Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

3' HIV LTR (3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3b_031

Plasmid

#123809

Purpose

Encodes Kruppel Associated Box repression domain fused to methyl CpG binding protein 2 as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

KRAB:meCP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Cx43K258stop-msfGFP

Plasmid

#69023

Purpose

Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx43 (Gja1 Rat)

Use

Tags

V206K monomerized super folder GFP

Expression

Mammalian

Mutation

delete codons 258-381 and fuse in frame to monome…

Promoter

CMV

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pB-Neo-rTOM20-GFP

Plasmid

#210331

Purpose

Overexpression of rTOM20

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rTOM20 (Tomm20 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-YdLight1

Plasmid

#125705

Purpose

Expresses the yellow genetically encoded dopamine sensor YdLight1 in mammalian cells.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Insert

YdLight1

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pKMD111e - intein-Cas9(S219-G521R)

Plasmid

#64192

Purpose

Expresses intein-Cas9(S219-G521R) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSIN-Skil-gRNA2

Plasmid

#180371

Purpose

targeting mouse Skil/SnoN gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Skil targeting gRNA (Skil Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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DT40

Plasmid

#80415

Purpose

Expresses 40 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 40 interrupted CTG repeats (DMPK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJB332

Plasmid

#115949

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 TetR-Gly9-DDX6 (contains SapI)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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