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Plasmid#72178PurposeExpresses the extracellular region of the Unc5B, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertUnc5b.a (Unc5b Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB3-mCherry-6xHis
Plasmid#130985PurposeBacterial expression of mouse EB3-mCherry-6xHis for protein purification.DepositorInsertEB3-mCherry-6xHis (Mapre3 Mouse)
UseTagsmCherry-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Eef25UTR-TOPmut-renilla
Plasmid#38236DepositorInsertEef2_TOPm_5UTR/Renilla (Eef2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationThe first nucleotides of the 5' UTR were cha…PromoterEndogenous Eef2Available sinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMM403_CaMKII-promoter
Plasmid#34926DepositorInsertCaMKII promoter (Camk2a Mouse)
UseMouse TargetingTagsExpressionMammalianMutationPromoterCaMKIIAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1
Plasmid#184397PurposeDonor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1DepositorInsertFirefly luciferase beta-globin-PTC39
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianMutationPTC in beta-globin at amino acid position 39PromoterTet-On 3GAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Jarid1c-IRES-GFP
Plasmid#166009PurposeRetroviral biscistronic expression of Jarid1c and eGFPDepositorInsertJarid1c (Kdm5c Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Cplx1-GFP KI
Plasmid#131475PurposeEndogenous tagging of Complexin1: C-terminal (amino acid position: L128)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-mAIM2-Flag
Plasmid#51537PurposeExpress Flag-tagged mAIM2 in mammalian cellsDepositorInsertAim2 (Aim2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
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LentiV_Neo_Sik3_T142Q
Plasmid#119973PurposeLentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance geneDepositorInsertSik3 (Sik3 Mouse)
UseLentiviralTagsExpressionMammalianMutationchange Threonine 142 to GlutaminePromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mouse-DJ1-HA
Plasmid#29396DepositorInsertDJ1 (Park7 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-sh-mFAK C
Plasmid#37015DepositorInsertsh-mFAK C (Ptk2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceMay 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
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MmSphk2
Plasmid#118598PurposeExpresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Opto-ACTU−
Plasmid#198869PurposeOptogenetic recruitment construct to increase inner membrane surface chargeDepositorInsertRad17 polyanionic tail (-14)
UseSynthetic BiologyTagsCRY2PHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shSMAD4 #1
Plasmid#83089PurposeLentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
ptdEos-CLC
Plasmid#38009DepositorInsertsUseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Aldh1L1 scFv [N103/31]
Plasmid#182080PurposeMammalian Expression of Aldh1L1 scFv. Derived from hybridoma N103/31.DepositorInsertrecombinant mouse scFv targeting Aldh1L1 (Rattus norvegicus) (Aldh1l1 Mouse)
UseTagsSortase, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4
Plasmid#58925PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only