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Plasmid#215826PurposeExpress CBE6c V106W (with SpCas9) in mammalian cellsDepositorInsertCBE6c V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
tFucci(SA)5
Plasmid#153520PurposeNew color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.DepositorInserttFucci(SA)5.
UseTagsAzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)ExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_TNF-SBP-mCherry
Plasmid#65279Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGM6
Plasmid#110660PurposeAdeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.DepositorInsertAAV6 cap genes, AAV2 rep genes, adenovirus helper genes
UseAAVTagsExpressionMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-BCKDK
Plasmid#23794DepositorInsertBCKDK (BCKDK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFMRPiso1
Plasmid#48690PurposeExpresses hFMRP iso 1DepositorInsertFlagHA FMR1 Iso 1 (FMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462) V2.0
Plasmid#62987PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-mCherry
Plasmid#36084Purpose3rd generation lentiviral plasmid expressing mCherryDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9-3XmCherry
Plasmid#64108PurposeExpression of Sp dCas9-3XmCherry in mammalian cellsDepositorInsertSp dCas9
UseCRISPR and LentiviralTags3XmCherryExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFH3.3_Cre-MS2x12
Plasmid#205559PurposeExpress Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertCre
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only