Showing: 301 - 320 of 362 results
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Plasmid#69025PurposeExpresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with sfGFP (V206-version) on the N-terminus. CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagssuper folder GFPExpressionMammalianMutationnon-monomerized sfGFP fused in frame to N-terminu…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDyn1
Plasmid#64067PurposeHuman cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).DepositorInsertDYNC1H1 (DYNC1H1 Human)
UseCre/LoxTagsHis-ZZ-TCS-SNAPfExpressionInsectMutationCodon optimized for Sf9PromoterAvailabilityAcademic Institutions and Nonprofits only -
DNA-CARzeta-IRESpuro
Plasmid#89345Purpose2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassetteDepositorInsertDNA-CARzeta
UseLentiviralTagsSNAPf tagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx43
Plasmid#69020PurposeExpresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagsEBFP2ExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx26
Plasmid#69021PurposeExpresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorUseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
msfGFP-Cx43
Plasmid#69024PurposeExpresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Cx43-msfGFP
Plasmid#69007PurposeExpresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertGja1 (Gja1 Rat)
UseTagsmonomerized superfolder GFPExpressionMammalianMutationPromotercmvAvailabilityAcademic Institutions and Nonprofits only -
Cx26-msfGFP
Plasmid#69016PurposeExpresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertCx26 (Gjb2 Rat)
UseTagsV206K monomerized super folder GFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx30
Plasmid#69022PurposeExpresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorInsertCx30 (GJB6 Human)
UseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Cx26-sfGFP
Plasmid#69026PurposeExpresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to non-monomerized superfolder GFP. Expression driven by CMV promoter.DepositorInsertCx26 (Gjb2 Rat)
UseTagsnon-monomerized superfolder GFP (V206)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Cx30-msfGFP
Plasmid#69019PurposeExpresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertCx30 (GJB6 Human)
UseTagsV206K monomerized super folder GFPExpressionMammalianMutationPromotercAvailabilityAcademic Institutions and Nonprofits only -
DNA-CARzeta-GFP
Plasmid#89344Purpose2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFPDepositorUseLentiviralTagsSNAPf tag and mEGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_MTX-LUCID
Plasmid#119047PurposePlasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_MTX-LUCID_part1
Fab_MTX-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Theo-LUCID
Plasmid#119048PurposePlasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Theo-LUCID_part1
Fab_Theo-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Quin-LUCID
Plasmid#119049PurposePlasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Quin-LUCID_part1
Fab_Quin-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailabilityAcademic Institutions and Nonprofits only -
pmVirD2
Plasmid#90314PurposemVirD2 expression plasmidDepositorInsertA. tumefaciens VirD2 from aa 1 to 204
UseTagsHis6x tagExpressionBacterialMutationPromoterT7/T5 IPTG inducibleAvailabilityAcademic Institutions and Nonprofits only -
pGEX-HA-GlpG
Plasmid#155135PurposeExpresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1DepositorInsertGlpG (glpG Escherichia coli)
UseTagsGST and HAExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pDC146
Plasmid#169366PurposeExpresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96DepositorInsertSyntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
UseTags6 his tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
ss-ED-mCherry-GPI
Plasmid#163680PurposeExprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cellsDepositorInsertThe luminal domain of ST6 beta-galactoside alpha-2,6-sialyltransferase 1 with N-terminally tagged signal sequences (ST6GAL1 Human)
UseTagsGPI anchor, mCherry, and signal sequencesExpressionMammalianMutationThis chimera contains the ecto-domain of ST. Its …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG
Plasmid#155131PurposeExpresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
UseTagsHisExpressionBacterialMutationinsert codes for GlpG residues 87-276PromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 301 - 320 of 362 results