pET15-His-∆NGlpG
(Plasmid #155131)

• Purpose: Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b
• Depositing Lab: Sinisa Urban
• Publication: Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910-918. doi: 10.1038/s41594-019-0296-9. Epub 2019 Sep 30.

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5700
• Total vector size (bp): 6340
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GlpG
• Species: Escherichia coli
• Insert Size (bp): 636
• Mutation: insert codes for GlpG residues 87-276
• GenBank ID: NC_000913.3
• Entrez Gene: glpG (a.k.a. b3424, ECK3410)
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter primer
• 3′ sequencing primer: T7 terminator primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry