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We narrowed to 384 results for: px458

Showing: 301 - 320 of 384 results

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p458 VQR

Plasmid

#101727

Purpose

Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Use

Tags

Expression

Mammalian

Mutation

VQR

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pDU1

Plasmid

#169817

Purpose

pSpCas9(BB)-2A-tCD19 (pDU1)

Depositor

Andrea Rossi

Article

Ramachandran et al Life Sci Alliance. 2021 Apr 26;4(6). pii

Insert

tCD19

Use

Tags

fusion protein

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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p183_LTJ_sgRNACD90.2_A

Plasmid

#82671

Purpose

sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD90.2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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p240_LTJ_sgRNAFoxp3K276X

Plasmid

#82675

Purpose

sgRNA targeting murine Foxp3 mutant. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting Foxp3 K276X

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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p204_LTJ_sgRNACD45.2_R2

Plasmid

#82673

Purpose

sgRNA targeting murine CD45.2 region 2. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD45.2 region 2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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p163_LTJ_sgRNACD90.1

Plasmid

#82677

Purpose

sgRNA targeting murine CD90.1. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting mouse CD90.1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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p206_LTJ_sgRNACD45.2_R3

Plasmid

#82674

Purpose

sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD45.2 region 3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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p458 VRER

Plasmid

#101728

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Use

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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p202_LTJ_sgRNACD45.2_R1

Plasmid

#82672

Purpose

sgRNA targeting murine CD45.2 region 1. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting mouse CD45.2 region 1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAS458-U6_CBA-mCherry

Plasmid

#195566

Purpose

Transient expression of mCherry; U6 with scaffold for sgRNA cloning in mammalian cells.

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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p236_LTJ_sgRNAFoxp3sf/J

Plasmid

#82676

Purpose

sgRNA targeting murine Foxp3 scurfy. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting Foxp3 sf/J

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mAMPKa1

Plasmid

#79004

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa1 (Prkaa1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
Cas9-GFP_sg_mTfeb

Plasmid

#79006

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

sgTfeb (Tfeb Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-tomato-TSC2-sgRNA

Plasmid

#196195

Purpose

Editing human TSC2 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

TSC2 (TSC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pQCi-mB2m-sgRNA

Plasmid

#154090

Purpose

pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA targeting murine B2M locus

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSCIPpay-BFP reporter

Plasmid

#154096

Purpose

Self-Cutting and Integrating Payload CRISPR backbone with BFP reporter

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

P2A-tagBFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgPYM1

Plasmid

#105249

Purpose

human PYM1 sgRNA

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

sgRNA for PYM1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only
Cas9-GFP_sg_mAMPKa2

Plasmid

#79005

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa2 (Prkaa2 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pMGS7 (AAVS1sgRNA)

Plasmid

#126582

Purpose

A CRISPR/Cas9 construct to target the AAVS1 locus in humans

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AAVS1 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

Plasmid

#107857

Purpose

To knock-out TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

gRNA against TARDBP (Exon 1) (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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